Department of Neuropathology, Institute of Pathology and Neuropathology, University Hospital of Tuebingen, Eberhard Karls University of Tuebingen, Tuebingen, Germany.
Department of Neurosurgery, Eberhard Karls University of Tuebingen, Baden-Württemberg, Germany.
J Clin Pathol. 2022 Nov;75(11):759-765. doi: 10.1136/jclinpath-2021-207526. Epub 2021 Jun 28.
An ependymoma shows divergent morphological and molecular features depending on their location. The paired box 6 () transcription factor is a putative tumour suppressor and drives cancer cells towards a stem cell-like state. A transcriptome study reported high expression in ependymal tumours, but data on protein expression are lacking.
We, therefore, analysed expression by immunohistochemistry in 172 ependymoma samples and correlated its expression to histology, WHO grade, anatomical location and molecular subgroups.
Mean nuclear expression in ependymoma was 27.5% (95% CI 23.3 to 31.7). expression in subependymoma (mean: 5%) was significantly lower compared with myxopapillary (30%), WHO grade II (26%) and anaplastic ependymoma (35%). Supratentorial ependymomas also displayed significant lower levels (15%) compared with spinal cord tumours (30%). Expression levels in YAP1-fused ependymoma (41%) were higher compared with REL-associated protein (RELA)-fusion positive tumours (17%), while expression was similar in posterior fossa group A (33%) and B (29%) ependymomas. Kaplan-Meier analysis in RELA-fusion positive ependymomas and posterior fossa group B showed a significant better outcome for at or above the cut-off of 19.45% compared with tumours with below the cut-off.
We demonstrate that is frequently expressed in human ependymal tumours and immunohistochemistry may be helpful in determining prognostic relevant subgroups.
室管膜瘤因其位置不同而呈现出不同的形态和分子特征。配对盒 6 () 转录因子是一种潜在的肿瘤抑制因子，可促使癌细胞向干细胞样状态发展。一项转录组研究报告称，在室管膜瘤中高表达，但缺乏关于蛋白表达的数据。
因此，我们通过免疫组织化学分析了 172 例室管膜瘤样本中的表达，并将其表达与组织学、世界卫生组织 (WHO) 分级、解剖位置和分子亚组相关联。
室管膜瘤的平均核表达为 27.5%（95%置信区间 23.3%至 31.7%）。与黏液乳头型（5%）相比，副神经节瘤（30%）、WHO 分级 II 级（26%）和间变性室管膜瘤（35%）的表达明显较低。与脊髓肿瘤（30%）相比，幕上室管膜瘤的表达水平也显著降低（15%）。YAP1 融合型室管膜瘤（41%）的表达水平高于 RELA 融合阳性肿瘤（17%），而在后颅窝 A 组（33%）和 B 组（29%）室管膜瘤中表达水平相似。RELA 融合阳性室管膜瘤和后颅窝 B 组的 Kaplan-Meier 分析显示，在截止值为 19.45%以上时，表达水平与肿瘤的预后相关，而低于截止值时则无明显相关性。
我们证明 广泛表达于人类室管膜瘤中，免疫组织化学检测可能有助于确定具有预后意义的亚组。
