School of Arts, Sciences and Humanities, University of São Paulo (USP), Arlindo Bettio Av., 1000, São Paulo 03828-000, Brazil.
Sao Carlos Institute of Physics, University of São Paulo (USP), Trabalhador São-Carlense Av., 400, Sao Carlos 13560-970, Brazil.
ACS Appl Mater Interfaces. 2021 Jul 14;13(27):31406-31417. doi: 10.1021/acsami.1c02686. Epub 2021 Jun 29.
The use of ultraviolet (UV) and blue irradiation to sterilize surfaces is well established, but commercial applications would be enhanced if the light source is replaced with ambient light. In this paper, it is shown that nanofibers can be explored as an alternative methodology to UV and blue irradiation for bacterial inactivation. It is demonstrated that this is indeed possible using spun nanofibers of poly[lactic--(glycolic acid)] (PLGA). This work shows that PLGA spun scaffolds can promote photoinactivation of and bacteria with ambient light or with laser irradiation at 630 nm. With the optimized scaffold composition of PLGA85:15 nanofibers, the minimum intensity required to kill the bacteria is much lower than in antimicrobial blue light applications. The enhanced effect introduced by PLGA scaffolds is due to their nanofiber structures since PLGA spun nanofibers were able to inactivate both and bacteria, but cast films had no effect. These findings pave the way for an entirely different method to sterilize surfaces, which is less costly and environmentally friendly than current procedures. In addition, the scaffolds could also be used in cancer treatment with fewer side effects since photosensitizers are not required.
利用紫外线（UV）和蓝光照射来对表面进行杀菌已经得到了广泛的认可，但如果将光源替换为环境光，商业应用将会得到增强。在本文中，我们表明可以探索纳米纤维作为替代 UV 和蓝光照射的细菌失活动力学方法。已经证明，使用聚乳酸-（乙醇酸）（PLGA）的纺制纳米纤维确实可以实现这一点。这项工作表明，PLGA 纺制支架可以使用环境光或在 630nm 激光照射下促进 和 细菌的光灭活。使用 PLGA85:15 纳米纤维的优化支架组成，杀死细菌所需的最小强度远低于抗菌蓝光应用。PLGA 支架引入的增强效应是由于它们的纳米纤维结构，因为 PLGA 纺制的纳米纤维能够使 和 细菌失活，但浇铸薄膜没有效果。这些发现为一种全新的表面杀菌方法铺平了道路，这种方法比目前的方法更经济、更环保。此外，由于不需要光敏剂，支架也可以用于癌症治疗，副作用更少。
