从大杂交鲟鱼软骨中分离的硫酸软骨素的表征及其对乙醇诱导的胃溃疡的胃保护活性。
Characterization of chondroitin sulfates isolated from large hybrid sturgeon cartilage and their gastroprotective activity against ethanol-induced gastric ulcers.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Quzhou Sturgeon Aquatic Food Science and Technology Development Co., Ltd., Quzhou 324002, China.
出版信息
Food Chem. 2021 Nov 30;363:130436. doi: 10.1016/j.foodchem.2021.130436. Epub 2021 Jun 20.
Sturgeon cartilage, which is rich in chondroitin sulfate (CS), is usually discarded during sturgeon utilization. In this paper, CS was isolated from large hybrid sturgeon skull and backbone and named SCS and BCS, respectively. Their structures were investigated via Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy and high performance liquid chromatography (HPLC). The average molecular weights of SCS and BCS were ~ 30-44 kDa. Disaccharide analysis indicated that SCS and BCS had similar chemical structures and were composed of ΔUA-[1 → 3]-GalNAc (ΔDi0S, 14.71%, 16.04%), ΔUA-[1 → 3]-GalNAc-4 s (ΔDi4S, 32.01%, 37.78%) and ΔUA-[1 → 3]-GalNAc-6 s (ΔDi6S, 53.27%, 46.18%). The gastroprotective effect of SCS and BCS were studied using a rat model of ethanol-induced gastric ulcers. Both SCS and BCS had apparent gastroprotective activity and their ulcer inhibition rate reached ~ 35%-45%, which was similar to that of omeprazole (~41%). These results provide useful strategies for the high-value utilization of sturgeon cartilage.
鲟鱼软骨富含硫酸软骨素（CS），在鲟鱼利用过程中通常被丢弃。本文分别从大杂交鲟鱼头骨和脊椎骨中分离出 CS，并将其命名为 SCS 和 BCS。通过傅里叶变换红外（FT-IR）光谱、核磁共振（NMR）光谱和高效液相色谱（HPLC）对其结构进行了研究。SCS 和 BCS 的平均分子量约为 30-44 kDa。二糖分析表明，SCS 和 BCS 具有相似的化学结构，由 ΔUA-[1→3]-GalNAc（ΔDi0S，14.71%，16.04%）、ΔUA-[1→3]-GalNAc-4s（ΔDi4S，32.01%，37.78%）和 ΔUA-[1→3]-GalNAc-6s（ΔDi6S，53.27%，46.18%）组成。采用乙醇诱导的大鼠胃溃疡模型研究了 SCS 和 BCS 的胃保护作用。SCS 和 BCS 均具有明显的胃保护活性，其溃疡抑制率达到约 35%-45%，与奥美拉唑（~41%）相似。这些结果为鲟鱼软骨的高值化利用提供了有用的策略。
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