School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, 212013, Zhenjiang, Jiangsu, China.
J Sci Food Agric. 2013 May;93(7):1633-40. doi: 10.1002/jsfa.5937. Epub 2013 Jan 3.
Chinese sturgeon (Acipenser sinensis), a rare species, is an important fishery resource in China. To save this species from overfishing, damming and pollution, artificial propagation has been developed rapidly in recent years. However, the cartilage from Chinese sturgeon, which contains an abundance of chondroitin sulfate (CS), is currently discarded as solid waste after consumption of the fish. The aim of this study was to investigate the extraction, purification and characterisation of CS from Chinese sturgeon cartilage.
The optimal extraction parameters were a ratio of aqueous NaOH to cartilage powder of 9.2, a concentration of aqueous NaOH of 4.4% and an extraction time of 3.9 h. Under these optimal conditions the yield of crude CS from Chinese sturgeon cartilage was 26.51%, in agreement with the model prediction (26.54%). Purification by DEAE-52 cellulose and Sephadex G-100 column chromatography yielded a single fraction, CS-11. Its characterisation indicated that CS-11 was consistent with the polysaccharide backbone structure -4GlcAβ-3GalNβ- and was present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate.
The results of this study provide a basis for promoting the utilisation of Chinese sturgeon resources and are significant for the development and utilisation of CS from Chinese sturgeon cartilage in the food industry.
中华鲟（Acipenser sinensis）是一种珍稀物种，是中国重要的渔业资源。为了防止该物种因过度捕捞、筑坝和污染而灭绝，近年来人工繁殖得到了迅速发展。然而，中华鲟的软骨在食用后，其中含有丰富的硫酸软骨素（CS），目前被当作固体废物丢弃。本研究旨在探讨从中华鲟软骨中提取、纯化和表征 CS 的方法。
最优提取参数为水合 NaOH 与软骨粉的比例为 9.2，水合 NaOH 的浓度为 4.4%，提取时间为 3.9 小时。在这些最优条件下，中华鲟软骨中粗 CS 的产率为 26.51%，与模型预测值（26.54%）一致。通过 DEAE-52 纤维素和 Sephadex G-100 柱层析纯化得到单一组分 CS-11。其特性表明 CS-11 与多糖主链结构-4GlcAβ-3GalNβ-一致，并且以软骨素-4-硫酸盐和软骨素-6-硫酸盐的形式存在。
本研究结果为促进中华鲟资源的利用提供了依据，对食品工业中开发和利用中华鲟软骨中的 CS 具有重要意义。
