Department of Agriculture and Environmental Sciences, National Institute of Food Technology Entrepreneurship and Management (NIFTEM), Kundli, Sonipat, Haryana 131028, India.
Department of Agriculture and Environmental Sciences, National Institute of Food Technology Entrepreneurship and Management (NIFTEM), Kundli, Sonipat, Haryana 131028, India.
Food Chem. 2021 Dec 1;364:130440. doi: 10.1016/j.foodchem.2021.130440. Epub 2021 Jun 23.
This study compared different extraction methods [sonication, alkaline hydrolysis, supramolecular solvent microextraction (SUPRAS) and Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS)] along with their greenness. An analytical method was validated for determination of USEPA's listed 16 polycyclic aromatic hydrocarbons' (PAHs) in cooked chicken and roasted coffee using high pressure liquid chromatography-fluorescence detector (HPLC-FLD) with a C18 column. The recoveries with QuEChERS ranged between 62.26 and 103.85% (except Naphthalene and Fluorene) and 52.63-78.69% (except Naphthalene) for chicken and coffee respectively while poor recoveries were observed with conventional methods. With SUPRAS, heavy PAHs' recovery in chicken was 71.33-112.23%. Limits of detection (LOD) were 0.03-0.06 ng/mL, regression coefficient values were 0.97-0.99 for 6.25-37.50 ng/mL quantification range. Relative standard deviation was found to be below 22%. The time and energy consumption per sample was 42.50 and 69.06 fold and 77.52 and 139.50 fold less with SUPRAS and QuEChERS method respectively as compared to alkaline hydrolysis.
本研究比较了不同的提取方法[超声处理、碱性水解、超分子溶剂微萃取（SUPRAS）和快速、简单、便宜、有效、坚固和安全（QuEChERS）]及其绿色性。采用高效液相色谱-荧光检测器（HPLC-FLD）和 C18 柱，建立了一种分析方法，用于测定烹饪鸡肉和烤咖啡中美国环保署（USEPA）列出的 16 种多环芳烃（PAHs）。QuEChERS 的回收率在鸡肉中为 62.26%至 103.85%（萘和芴除外）和 52.63%至 78.69%（萘除外），而传统方法的回收率则较差。采用 SUPRAS，鸡肉中重多环芳烃的回收率为 71.33%至 112.23%。检测限（LOD）为 0.03-0.06 ng/mL，6.25-37.50 ng/mL 定量范围内的回归系数值为 0.97-0.99。相对标准偏差低于 22%。与碱性水解相比，SUPRAS 和 QuEChERS 方法的每个样品的时间和能量消耗分别减少了 42.50 倍和 69.06 倍，以及 77.52 倍和 139.50 倍。
