Chuah M I, Au C
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T.
J Neurosci Res. 1988 May;20(1):84-9. doi: 10.1002/jnr.490200112.
In vitro studies on E15 rat embryos have shown that the number of receptor neurons containing olfactory marker protein (OMP) is markedly increased when olfactory mucosa (OM) is cultured in direct contact with the presumptive olfactory bulb (POB). This facilitatory influence is tissue-specific; that is, it is absent when the POB is substituted with other nervous or non-nervous tissues. In the present quantitative immunohistochemical study, we show that the enhancing influence of the POB is also a stage-specific phenomenon. The number of OMP-containing receptor cells is greatest when E15 OM is cultured with POB of the same age. If the POB was taken from a less mature (E13) or from a more mature (E17) embryo, the number of OMP-containing cells was greatly reduced.
对E15大鼠胚胎的体外研究表明，当嗅黏膜（OM）与假定的嗅球（POB）直接接触培养时，含有嗅觉标记蛋白（OMP）的受体神经元数量会显著增加。这种促进作用具有组织特异性；也就是说，当用其他神经或非神经组织替代POB时，这种作用就不存在了。在本定量免疫组织化学研究中，我们表明POB的增强作用也是一种阶段特异性现象。当E15 OM与相同年龄的POB一起培养时，含OMP的受体细胞数量最多。如果POB取自不太成熟（E13）或更成熟（E17）的胚胎，含OMP的细胞数量会大大减少。
