Gilroy D, Shaw C, Parry E, Odling-Smee W
Department of Surgery, Queen's University of Belfast, Northern Ireland.
J Trauma. 1988 Sep;28(9):1312-6. doi: 10.1097/00005373-198809000-00003.
Polymerised pyridoxylated haemoglobin solution (PPSFH) is a modified haemoglobin solution which has a normal oxygen carrying capacity, long half-life, and reasonable oxygen affinity, and is a leading candidate for use as an oxygen carrying blood substitute. Previous work has given indications that vasoactive factors may be present. A bioassay sensitive to vasoconstrictors was constructed. PPSFH prepared by chloroform extraction produced a mean pressure rise of 24.3 mm Hg. PPSFH prepared by a crystallisation method showed a mean rise of 2 mm Hg (p = 0.0004). Unmodified stroma-free haemoglobin (SFH) prepared from platelet- and white cell-free red blood cells showed a mean rise of 32.5 mm Hg. These data indicate that both PPSFH and SFH contain a vasoconstrictor factor which is of low molecular weight, is hydrophilic, and is derived from red blood cells.
聚合吡哆醛化血红蛋白溶液（PPSFH）是一种经过改良的血红蛋白溶液，具有正常的携氧能力、较长的半衰期和合理的氧亲和力，是用作携氧血液替代品的主要候选物。先前的研究表明可能存在血管活性因子。构建了一种对血管收缩剂敏感的生物测定法。通过氯仿萃取制备的PPSFH使平均血压升高24.3毫米汞柱。通过结晶法制备的PPSFH平均升高2毫米汞柱（p = 0.0004）。从无血小板和无白细胞的红细胞制备的未修饰无基质血红蛋白（SFH）平均升高32.5毫米汞柱。这些数据表明，PPSFH和SFH都含有一种血管收缩因子，该因子分子量低、亲水性强且源自红细胞。
