Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu 200014, People's Republic of China.
J Genet. 2021;100.
Drought limits the pear yield and quality. The birch-leaf pear ( Bunge) is one of the most frequently used pear rootstocks. Identifying genes involved in drought resistance of would suggest candidate genes for molecular breeding. We used single-molecule long-read sequencing technology to investigate the transcriptome of birch-leaf pear under drought stress. As a result, 362,139 consensus reads were identified using six databases, among which 342,162 genes were functionally annotated. Further, we identified 7094 long noncoding RNAs. The sequencing data contained 9891 alternative splicing and 100,836 alternative polyadenylation events. We report here the full-length sequence of birch-leaf pear, which can be used for breeding enhanced varieties.
干旱限制了梨的产量和品质。山梨（Bunge）是最常用的梨砧木之一。鉴定与抗旱性相关的基因将为分子育种提供候选基因。我们使用单分子长读测序技术研究了干旱胁迫下山梨的转录组。结果，使用六个数据库鉴定了 362139 条一致reads，其中 342162 个基因具有功能注释。此外，我们还鉴定了 7094 个长非编码 RNA。测序数据包含 9891 个选择性剪接和 100836 个选择性多聚腺苷酸化事件。我们在这里报告了山梨的全长序列，可用于培育改良品种。
