Shi Lei, Wang Kexin, Yu Jinghong, Li Mingkai, Men Guangmei, Ma Gang, Wang Xing
Radiology Department, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010020 Inner Mongolia China.
Joint Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010020 Inner Mongolia China.
Indian J Orthop. 2020 Oct 29;55(4):974-982. doi: 10.1007/s43465-020-00293-2. eCollection 2021 Aug.
To investigate the relationship between quantitative analysis of MRI (T2-mapping) and the expression of matrix metalloproteinase (MMP-1, MMP-3) in osteoarthritis of the knee joint and the role of MMP-1,3 in the pathogenesis of osteoarthritis.
Thirty cases of knee osteoarthritis (KOA) patients with total knee arthroplasty (TKA) (lesion group) and 30 healthy adult volunteers (control group) were scanned with 1.5 T routine MR and T2-mapping, and their T2 values were measured and statistically analyzed. The pathological examination of the knee cartilage that was replaced during the operation and the immunohistochemical assay were used to measure the expression of MMP-1,3. The correlation between the T2 value of magnetic resonance imaging and the expression of MMP-1,3 was analyzed.
(1) According to the Recht grading standard for magnetic resonance, the T2 value of magnetic resonance increased significantly with the increase of cartilage degeneration. The differences in T2 values between each level and the normal group were statistically significant ( < 0.05). (2) The T2 value of magnetic resonance imaging increased with the severity of the cartilage degeneration pathological Mankin grading, and the difference was statistically significant ( < 0.05). (3) The expression of MMP-1,3 increased with cartilage degeneration. (4) The T2 value and the expression of MMP-1 in cartilage showed a linear trend. The result of Spearman correlation analysis showed that the expression of MMP-1,3 increased as the cartilage T2 value increased. There was a positive linear correlation between the two.
The T2 value of magnetic resonance increased with the degeneration of KOA cartilage. The expression of MMP-1,3 increased with the severity of articular cartilage destruction. The T2 value of KOA magnetic resonance was positively correlated with the expression of MMP-1,3.
探讨膝关节骨关节炎中MRI定量分析（T2映射）与基质金属蛋白酶（MMP-1、MMP-3）表达之间的关系，以及MMP-1、3在骨关节炎发病机制中的作用。
对30例行全膝关节置换术（TKA）的膝关节骨关节炎（KOA）患者（病变组）和30名健康成年志愿者（对照组）进行1.5T常规MR及T2映射扫描，测量其T2值并进行统计学分析。对术中切除的膝关节软骨进行病理检查及免疫组化检测，以测量MMP-1、3的表达。分析磁共振成像T2值与MMP-1、3表达之间的相关性。
（1）根据磁共振Recht分级标准，磁共振T2值随软骨退变程度增加而显著升高。各等级与正常组之间T2值差异有统计学意义（<0.05）。（2）磁共振成像T2值随软骨退变病理Mankin分级严重程度增加而升高，差异有统计学意义（<0.05）。（3）MMP-1、3表达随软骨退变程度增加而升高。（4）软骨中T2值与MMP-1表达呈线性趋势。Spearman相关性分析结果显示，随着软骨T2值升高，MMP-1、3表达增加。两者呈正线性相关。
KOA软骨退变时磁共振T2值升高。MMP-1、3表达随关节软骨破坏严重程度增加而升高。KOA磁共振T2值与MMP-1、3表达呈正相关。
