Department of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Fauji Foundation Hospital, Rawalpindi, Pakistan.
Future Oncol. 2021 Sep;17(27):3561-3577. doi: 10.2217/fon-2020-1155. Epub 2021 Jun 30.
The present study was planned to explore the expression variations of mitochondrial sirtuins and the mitochondrial DNA repair enzyme in leukemia patients. Oxidative stress and deacetylation levels of leukemia patients were measured in the present study. A total of 200 leukemia patients along with 200 healthy controls were evaluated using quantitative PCR, 8OXOG assay and deacetylation assay. Significant deregulation of (p < 0.0001), (p < 0.0001), (p < 0.0001), (p < 0.0001) and (p < 0.0001) was detected in patients versus controls. Survival analysis showed that deregulation of said genes was associated with decreased survival of leukemia patients (: p < 0.004; : p < 0.0009; : p < 0.0001; : p < 0.03). Receiver operating characteristic curve analysis confirmed the diagnostic values of selected genes in leukemia patients. Levels of 8OXOG adducts were measured, and significantly increased 8OXOG adduct levels were observed in patients versus controls. These data suggest that deregulation of , , and acts as a diagnostic and prognostic marker in leukemia.
本研究旨在探索白血病患者中线粒体去乙酰化酶和线粒体 DNA 修复酶的表达变化。本研究测量了白血病患者的氧化应激和去乙酰化水平。使用定量 PCR、8OXOG 测定和去乙酰化测定评估了 200 例白血病患者和 200 例健康对照者。与对照组相比，患者中 (p < 0.0001)、 (p < 0.0001)、 (p < 0.0001)、 (p < 0.0001) 和 (p < 0.0001) 的表达显著失调。生存分析表明，这些基因的失调与白血病患者生存率降低有关（: p < 0.004；: p < 0.0009；: p < 0.0001；: p < 0.03）。受试者工作特征曲线分析证实了所选基因在白血病患者中的诊断价值。还测量了 8OXOG 加合物的水平，与对照组相比，患者中观察到 8OXOG 加合物水平显著升高。这些数据表明， 、 、 和 的失调可作为白血病的诊断和预后标志物。
