Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, China.
School of Public Health and Family Medicine, Capital Medical University, Beijing, China.
J Food Sci. 2021 Aug;86(8):3457-3466. doi: 10.1111/1750-3841.15819. Epub 2021 Jun 30.
Shellfish allergies constitute an important cause of food-induced anaphylactic reactions, which pose challenges to food safety and human health worldwide. In the present study, the specific IgE (sIgE) binding characteristics of different shrimp proteins of black tiger shrimp (Penaeus monodon) to the sera of eight shrimp-allergic patients from China were studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and nanoliquid chromatography time-of-flight mass spectrometry. According to the PLGS scores (>2000) and the sequence coverage (>40%), eight proteins with sIgE binding activity were identified, including myosin heavy chain type 1 (K4Q4N8), hemocyanin (G1AP69 and Q95V28), phosphopyruvate hydratase (O96656), arginine kinase (C7E3T4), tropomyosin (A1KYZ2), sarcoplasmic calcium binding protein (H7CHW2) and glyceraldehyde-3-phosphate dehydrogenase (A0A097BQP2). Among these eight proteins, phosphopyruvate hydratase was a prevalent IgE-binding protein among these Chinese patients with binding observed in 100% of sera. Moreover, 13 peptides were predicted as epitopes of phosphopyruvate hydratase. These new details help us to understand the crustacean IgE-binding proteins especially Penaeus monodon IgE-binding proteins, that would cause allergic reaction to Chinese patients. And our findings may provide essential information to improve allergy prevention and clinical treatment to shrimp allergy in China. PRACTICAL APPLICATION: This research may have diagnostic and therapeutic value for shrimp allergies in China.
贝类过敏是食物引起过敏反应的一个重要原因，对全球食品安全和人类健康构成挑战。本研究采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和纳流色谱-飞行时间质谱联用技术，研究了黑虎虾（Penaeus monodon）不同虾蛋白与 8 例中国虾过敏患者血清的特异性 IgE（sIgE）结合特性。根据 PLGS 评分（>2000）和序列覆盖率（>40%），鉴定出 8 种具有 sIgE 结合活性的蛋白，包括肌球蛋白重链 1 型（K4Q4N8）、血蓝蛋白（G1AP69 和 Q95V28）、磷酸烯醇丙酮酸水合酶（O96656）、精氨酸激酶（C7E3T4）、原肌球蛋白（A1KYZ2）、肌钙蛋白（H7CHW2）和甘油醛-3-磷酸脱氢酶（A0A097BQP2）。在这 8 种蛋白中，磷酸烯醇丙酮酸水合酶是中国患者中普遍存在的 IgE 结合蛋白，100%的血清中均观察到其结合。此外，预测了 13 个肽段为磷酸烯醇丙酮酸水合酶的表位。这些新的细节有助于我们了解甲壳类 IgE 结合蛋白，特别是对引起中国患者过敏反应的黑虎虾 IgE 结合蛋白。我们的研究结果可为中国虾过敏的过敏预防和临床治疗提供重要信息。
本研究对中国的虾过敏可能具有诊断和治疗价值。
