高渗葡萄糖（10%）在预防大鼠术后粘连中的应用。

Use of hypertonic glucose (10%) in the prevention of postoperative adhesions in rats.

机构信息

PhD. Universidade Federal do Maranhão - College of Medicine - Department of Medicine I - São Luís (MA), Brazil.

PhD. Universidade Federal do Maranhão - College of Medicine - Department of Medicine II - São Luís (MA), Brazil.

出版信息

Acta Cir Bras. 2021 Jun 25;36(5):e360504. doi: 10.1590/ACB360504. eCollection 2021.

DOI:10.1590/ACB360504

PMID:34190774

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8232060/

Abstract

PURPOSE

To evaluate the efficacy of hypertonic glucose (10%), alone or in combination with the corticoid dexamethasone, to prevent peritoneal adhesion following hysterectomy in rats.

METHODS

Forty-two adult rats underwent hysterectomy with peritoneal lavage: G1 - glucose (10%); G2 - glucose (10%) and dexamethasone 3 mg·kg-1; and G3 - physiological saline (PS) 0.9%.

RESULTS

In the macroscopic analysis after 14 days, G1 had a median score of 1, G2 of 1, and G3 of 2.5 (p < 0.0001), G3 compared to G1 and G2. There was no difference between groups after 28 days. In the microscopic analysis, the median vascular proliferation after 14 days was 2 for G1, 1 for G2, and 3 for G3 (p = 0.0037, G3 vs. G1 and G2). After 28 days, G1 showed a median vascular proliferation score of 2, G2 of 2.5, and G3 of 3 (p < 0.0001, G3 vs. G1 and G2). Regarding the inflammatory reaction after 14 days, G1 had a median score of 2, G2 of 1, and G3 of 3 (p = 0.7916). After 28 days, G1 had a median score of 0.5 (0-1.75), G2 of 1.5, and G3 of 2.5 (p < 0.0001, G3 vs. the others and G2 vs. G1). In the evaluation of fibrosis after 14 days, G1 had a median score of 1, G2 of 1, and G3 of 2.5 (p < 0.0001, G3 vs. G1and G2). After 28 days, G1 had a median fibrosis score of 1, G2: 2, and G3: 2.5 (p < 0.0001), G3 vs. the others andG2 vs. G1).

CONCLUSIONS

The use of hypertonic glucose (10%) solution seems to reduce macroscopic and microscopic pelvic adhesions.

摘要

目的

评估高渗葡萄糖（10%）单独或联合皮质类固醇地塞米松预防大鼠子宫切除术后腹膜粘连的疗效。

方法

42 只成年大鼠行子宫切除术并进行腹腔冲洗：G1-葡萄糖（10%）；G2-葡萄糖（10%）和地塞米松 3mg·kg-1；G3-生理盐水（PS）0.9%。

结果

14 天后的大体分析中，G1 的中位数评分为 1，G2 为 1，G3 为 2.5（p<0.0001），G3 与 G1 和 G2 相比。28 天后各组之间无差异。在 14 天的微血管增生分析中，G1 的中位数为 2，G2 为 1，G3 为 3（p=0.0037，G3 与 G1 和 G2 相比）。28 天后，G1 的微血管增生评分中位数为 2，G2 为 2.5，G3 为 3（p<0.0001，G3 与 G1 和 G2 相比）。关于 14 天后的炎症反应，G1 的中位数评分为 2，G2 为 1，G3 为 3（p=0.7916）。28 天后，G1 的中位数评分为 0.5（0-1.75），G2 为 1.5，G3 为 2.5（p<0.0001，G3 与其他组相比，G2 与 G1 相比）。在 14 天的纤维化评估中，G1 的中位数评分为 1，G2 为 1，G3 为 2.5（p<0.0001，G3 与 G1 和 G2 相比）。28 天后，G1 的纤维化评分中位数为 1，G2 为 2，G3 为 2.5（p<0.0001），G3 与其他组相比，G2 与 G1 相比）。