瘤内注射康莱特治疗大鼠移植性肝癌的疗效

Efficacy of intra-tumor injection of Kang-Lai-Te in treating transplanted hepatoma in rats.

作者信息

Wu Li-Qun, Lu Yun, Lu Hua-Jun, Zhao Zong-Gang, Yang Meng

机构信息

Department of Hepato-biliary-vascular Surgery, Qingdao University Medical College, Qingdao 266003, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2004 Nov;3(4):580-4.

PMID:15567749

Abstract

BACKGROUND

Non-operative therapy takes an important position in comprehensive therapy of liver cancer. Despite some effects by using ethanol, acetic acid and heat saline for intra-tumor injection in the treatment of liver cancer, it is difficult to attain a complete cure but bring about injury to the liver to some extent. Hence, searching for other drugs for the local treatment of liver tumor is an important option. This study was designed to set up rat models of transplanted liver cancer, intra-tumor injection of Kang-Lai-Te (KLT), and negative control (saline) and positive control (ethanol). The effect of intra-tumor injection of KLT in treating transplanted hepatoma in rats and its advantages and disadvantages were assessed and the possibility of its use in treating patients with liver cancer was evaluated.

METHODS

Forty rats were divided into 4 groups (G1, G2, G3 and G4, 10 rats in each group). Different drugs were injected into their implanted hepatoma (G1 with 0.2 ml saline as control, G2 with 10 mg KLT, G3 with 20 mg KLT, G4 with 0.2 ml ethanol). After 3 and 8 days, the hepatoma volume (HV), the serum levels of albumin, alanine aminotransferase(ALT), aspartate aminotransferase alkaline phosphatase(ALP) and creatinine, as well as the expression of proliferation cell nuclear antigen(PCNA) in hepatoma were detected.

RESULTS

After 3 days, the HVs were smaller in G3 and G4 than in G1 (P<0.05), the serum levels of albumin were higher in G2 and G3 than in G1 and G4 (P<0.05), the serum levels of ALT and AST were lower in G2 and G3 than in G4 (P<0.05), the serum levels of ALP was lower in G2 and G3 than in G1 and G4 (P<0.05),the PCNA labeling indexes (PCNA LI) were lower in G2 and G3 than in G1 and G4 (P<0.05). After 8 days, the HVs were smaller in G2, G3 and G4 than in G1 (P<0.05), and the differences of HVs among G2, G3 and G4 were not significant. The serum levels of ALP were lower in G1, G2 and G3 than in G4 (P<0.05), and the PCNA LI were lower in G3 than in G1 and G4 (P<0.05).

CONCLUSION

Intra-tumor injection of KLT into implanted hepatoma is evidently effective, but it is less effective than ethanol. The effect of KLT on liver function is markedly lower than that of ethanol.

摘要

背景

非手术治疗在肝癌综合治疗中占据重要地位。尽管使用乙醇、乙酸和热盐水进行瘤内注射治疗肝癌有一定效果，但难以实现完全治愈，且会在一定程度上对肝脏造成损伤。因此，寻找其他用于肝脏肿瘤局部治疗的药物是一个重要选择。本研究旨在建立大鼠移植性肝癌模型，瘤内注射康莱特（KLT），并设置阴性对照（生理盐水）和阳性对照（乙醇）。评估瘤内注射KLT治疗大鼠移植性肝癌的效果及其优缺点，并评估其用于治疗肝癌患者的可能性。

方法

40只大鼠分为4组（G1、G2、G3和G4，每组10只）。将不同药物注射到其植入的肝癌中（G1注射0.2 ml生理盐水作为对照，G2注射10 mg KLT，G3注射20 mg KLT，G4注射0.2 ml乙醇）。在3天和8天后，检测肝癌体积（HV）、血清白蛋白、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶、碱性磷酸酶（ALP）和肌酐水平，以及肝癌中增殖细胞核抗原（PCNA）的表达。

结果

3天后，G3和G4组的HV小于G1组（P<0.05），G2和G3组的血清白蛋白水平高于G1和G4组（P<0.05），G2和G3组的血清ALT和AST水平低于G4组（P<0.05），G2和G3组的血清ALP水平低于G1和G4组（P<0.05），G2和G3组的PCNA标记指数（PCNA LI）低于G1和G4组（P<0.05）。8天后，G2、G3和G4组的HV小于G1组（P<0.05），G2、G3和G4组之间的HV差异不显著。G1、G2和G3组的血清ALP水平低于G4组（P<0.05），G3组的PCNA LI低于G1和G4组（P<0.05）。