Government College University, Department of Biochemistry, Faisalabad, Pakistan.
Government College University, Department of Microbiology, Faisalabad, Pakistan.
Braz J Biol. 2021 Jun 28;82:e239991. doi: 10.1590/1519-6984.239991. eCollection 2021.
High resistance to antimicrobials is associated with biofilm formation responsible for infectious microbes to withstand severe conditions. Therefore, new alternatives are necessary as biofilm inhibitors to control infections. In this study, the antimicrobial and antibiofilm activities of Fagonia indica extracts were evaluated against MDR clinical isolates. The extract exhibited its antibiofilm effect by altering adherence and disintegration of bacterial cell wall. Fagonia indica has antibacterial effect as minimum inhibitory concentration (MIC) values ranging from 125 to 500 µg mL-1 and minimum bactericidal concentration (MBC) value was 500-3000 µg mL-1 against multidrug resistant (MDR) clinical isolates. The extract exhibited its antibiofilm effect by altering adherence and disintegration of bacterial cell wall. Fagonia indica had antibacterial effect as minimum inhibitory concentration (MIC) values ranging from 125 to 500 µg mL-1 and minimum bactericidal concentration (MBC) value was 500-3000 µg mL-1 against MDR isolates. The maximum inhibitory effects of Fagonia indica chloroform extract on biofilm formation was observed on Staphylococcus aureus (71.84%) followed by Klebsiella pneumoniae (70.83%) after 48 hrs showing that inhibition is also time dependent. Our results about bacterial cell protein leakage indicated that MDR isolates treated with chloroform extract of Fagonia indica showed maximum protein leakage of K. pneumoniae (59.14 µg mL-1) followed by S. aureus (56.7 µg mL-1). Cell attachment assays indicated that chloroform extract resulted in a 43.5-53.5% inhibition of cell adherence to a polystyrene surface. Our results revealed that extracts of Fagonia indica significantly inhibited biofilm formation among MDR clinical isolates, therefore, could be applied as antimicrobial agents and cost effective biofilm inhibitor against these MDR isolates.
高耐药性与生物膜形成有关，生物膜使感染微生物能够耐受恶劣环境。因此，有必要寻找新的替代品作为生物膜抑制剂来控制感染。在这项研究中，评估了印度榕提取物对多药耐药临床分离株的抗菌和抗生物膜活性。提取物通过改变细菌细胞壁的黏附和崩解来发挥其抗生物膜作用。印度榕提取物对多药耐药（MDR）临床分离株的最小抑菌浓度（MIC）值范围为 125-500μg mL-1，最小杀菌浓度（MBC）值为 500-3000μg mL-1，表现出抗菌作用。提取物通过改变细菌细胞壁的黏附和崩解来发挥其抗生物膜作用。印度榕提取物对多药耐药（MDR）临床分离株的最小抑菌浓度（MIC）值范围为 125-500μg mL-1，最小杀菌浓度（MBC）值为 500-3000μg mL-1，表现出抗菌作用。印度榕氯仿提取物对生物膜形成的最大抑制作用在金黄色葡萄球菌（71.84%）中观察到，48 小时后肺炎克雷伯菌（70.83%），这表明抑制作用也是时间依赖性的。我们关于细菌细胞蛋白渗漏的结果表明，用印度榕氯仿提取物处理的多药耐药分离株表现出肺炎克雷伯菌（59.14μg mL-1）最大的蛋白渗漏，其次是金黄色葡萄球菌（56.7μg mL-1）。细胞附着试验表明，氯仿提取物导致细胞对聚苯乙烯表面的附着抑制了 43.5-53.5%。我们的结果表明，印度榕提取物显著抑制了多药耐药临床分离株的生物膜形成，因此，可作为抗微生物剂和具有成本效益的生物膜抑制剂应用于这些多药耐药分离株。
