Herness M S
Laboratory of Neurobiology and Behavior, Rockefeller University, New York, NY 10021.
J Neurosci Methods. 1988 Jul;24(3):237-42. doi: 10.1016/0165-0270(88)90168-9.
A new stimulating technique for studying the gustatory sensitivity of the glossopharyngeal nerve is presented. Among its other functions the glossopharyngeal nerve relays multisensory information from the posterior tongue region. Study of the gustatory function of this nerve is therefore complicated by concomitant tactile and thermal components. Additionally taste buds innervated by the glossopharyngeal nerve are located in trenches within the foliate and circumvallate papillae which results in slow delivery and removal of tastant solutions. By delivering the stimulus or adapting solution through an infusion pump connected to a double-barrelled pipette placed into the trench of the circumvallate papilla, quick delivery and removal of the stimulus is possible. Moreover the continuous flow of solution, switched from adapting solution to stimulus and back, allows tactile components to completely adapt. Due to both the proximity of the stimulus to the receptors and the continuous flow of the solution this technique produces gustatory responses with large phasic components, as is commonly observed in other gustatory nerves. The temporal characteristics of the gustatory portion of the glossopharyngeal nerve can therefore now be more readily studied.
本文介绍了一种用于研究舌咽神经味觉敏感性的新刺激技术。舌咽神经的其他功能之一是传递来自舌后区域的多感觉信息。因此，该神经味觉功能的研究因伴随的触觉和热觉成分而变得复杂。此外，由舌咽神经支配的味蕾位于叶状乳头和轮廓乳头的沟内，这导致味觉剂溶液的递送和去除缓慢。通过连接到置于轮廓乳头沟内的双管移液器的输液泵来递送刺激物或适应溶液，可以快速递送和去除刺激物。此外，溶液从适应溶液切换到刺激物再切换回适应溶液的连续流动，使触觉成分能够完全适应。由于刺激物与感受器接近以及溶液的连续流动，该技术产生具有大时相成分的味觉反应，这在其他味觉神经中很常见。因此，现在可以更容易地研究舌咽神经味觉部分的时间特征。
