Laboratoire des IMRCP, Université de Toulouse, CNRS UMR 5623, Université Toulouse III - Paul Sabatier, Toulouse, France.
Laboratoire des IMRCP, Université de Toulouse, CNRS UMR 5623, Université Toulouse III - Paul Sabatier, Toulouse, France.
J Colloid Interface Sci. 2021 Dec;603:333-343. doi: 10.1016/j.jcis.2021.06.058. Epub 2021 Jun 11.
Recently, a low molecular weight hydrogel based on a carbohydrate alkyl amide has been successfully used as biomaterial for neuron cell culture and for 3D printing. Varying the molecular structure should make it possible to extend the library of carbohydrate low molecular weight hydrogels available for these applications and to improve their performances.
Thirteen molecules easy to synthetize and designed to be potentially biocompatible were prepared. They are based on gluconamide, glucoheptonamide, galactonamide, glucamide, aliphatic chains and glycine. Their gelation in water was investigated in thermal conditions and wet spinning conditions, namely by dimethylsulfoxide-water exchange under injection.
Nine molecules give hydrogels in thermal conditions. By wet spinning, six molecules self-assemble fast enough, within few seconds, to form continous hydrogel filaments. Therefore, the method enables to shape by injection these mechanically fragile hydrogels, notably in the perspective of 3D printing. Depending on the molecular structure, persistent or soluble gel filaments are obtained. The microstructures are varied, featuring entangled ribbons, platelets or particles. In thermal gelation, molecules with a symmetrical polar head (galacto, glucoheptono) give flat ribbons and molecules with an asymmetrical polar head (gluco) give helical ribbons. The introduction of an extra glycine linker disturbs this trend.
最近，一种基于碳水化合物烷基酰胺的低分子量水凝胶已成功用作神经元细胞培养和 3D 打印的生物材料。改变分子结构应该可以扩展可用于这些应用的碳水化合物低分子量水凝胶库，并提高它们的性能。
制备了 13 种易于合成且设计为潜在生物相容的分子。它们基于葡糖酰胺、葡庚酰胺、半乳糖酰胺、葡酰胺、脂肪链和甘氨酸。研究了它们在热条件下和湿纺条件下水凝胶的形成，即在注射下通过二甲亚砜-水交换。
在热条件下，有 9 种分子形成水凝胶。通过湿纺，有 6 种分子能够在几秒钟内快速自组装形成连续的水凝胶纤维。因此，该方法能够通过注射来成型这些机械脆弱的水凝胶，特别是在 3D 打印方面。根据分子结构，可以获得持久或可溶性的凝胶纤维。微观结构不同，具有缠结的带、板或颗粒。在热凝胶化中，具有对称极性头（半乳糖、葡庚糖）的分子形成扁平带，而具有不对称极性头（葡糖）的分子形成螺旋带。引入额外的甘氨酸连接子会破坏这种趋势。
