Improved detection by time-resolved fluorometry of specific DNA immobilized in microtiter wells with europium/metal-chelator labelled DNA probes.
作者信息
Oser A, Valet G
机构信息
Mildred-Scheel-Labor für Krebszellforschung, Max-Planck-Institut für Biochemie, Martinsried, FRG.
出版信息
Nucleic Acids Res. 1988 Aug 25;16(16):8178. doi: 10.1093/nar/16.16.8178.
Abstract
摘要
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Improved detection by time-resolved fluorometry of specific DNA immobilized in microtiter wells with europium/metal-chelator labelled DNA probes.通过时间分辨荧光法利用铕/金属螯合剂标记的DNA探针改进对固定在微量滴定孔中的特定DNA的检测。
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Preparation of europium-labelled DNA probes and their properties.铕标记DNA探针的制备及其性质
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Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples.夹心杂交作为一种检测粗样品中核酸的便捷方法。
Gene. 1983 Jan-Feb;21(1-2):77-85. doi: 10.1016/0378-1119(83)90149-x.
2
Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.使用螯合基团取代补骨脂素标记的DNA探针进行灵敏的非放射性斑点杂交以及通过铕离子荧光进行定量检测。
Nucleic Acids Res. 1988 Feb 11;16(3):1181-96. doi: 10.1093/nar/16.3.1181.
3
Quantification of picogram levels of specific DNA immobilized in microtiter wells.对固定在微量滴定板孔中的特定皮克级DNA进行定量分析。
FEBS Lett. 1985 Apr 22;183(2):379-82. doi: 10.1016/0014-5793(85)80814-0.
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