Lim Hye-Sun, Yoon Kyeong-No, Chung Jin Ho, Lee Yong-Seok, Lee Dong Hun, Park Gunhyuk
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju 58245, Korea.
Department of Biomedical Sciences, Graduate School, Seoul National University, Seoul 03080, Korea.
Antioxidants (Basel). 2021 Jun 7;10(6):920. doi: 10.3390/antiox10060920.
Ultraviolet (UV) radiation has a strong biological effect on skin biology, and it switches on adaptive mechanisms to maintain homeostasis in organs such as the skin, adrenal glands, and brain. In this study, we examined the adaptation of the body to repeated bouts of UVB radiation, especially with respect to the catecholamine synthesis pathway of the adrenal glands. The effects of UVB on catecholamine-related enzymes were determined by neurochemical and histological analyses. To evaluate catecholamine changes after chronic excessive UVB irradiation of mouse skin, we examined dopamine and norepinephrine levels in the adrenal glands and blood from UV-irradiated and sham-irradiated mice. We found that chronic excessive UVB exposure significantly reduced dopamine levels in both tissues but did not affect norepinephrine levels. In addition, UVB irradiation significantly increased the levels of related enzymes tyrosine hydroxylase and dopamine-β-hydroxylase. Furthermore, we also found that apoptosis-associated markers were increased and that oxidative defense proteins were decreased, which might have contributed to the marked structural abnormalities in the adrenal medullas of the chronically UVB-irradiated mice. This is the first evidence of the damage to the adrenal gland and subsequent dysregulation of catecholamine metabolism induced by chronic exposure to UVB.
紫外线（UV）辐射对皮肤生物学具有强烈的生物学效应，它会开启适应性机制以维持皮肤、肾上腺和大脑等器官的内环境稳定。在本研究中，我们研究了机体对反复多次紫外线B（UVB）辐射的适应性，特别是关于肾上腺的儿茶酚胺合成途径。通过神经化学和组织学分析确定UVB对儿茶酚胺相关酶的影响。为了评估小鼠皮肤长期过度暴露于UVB后的儿茶酚胺变化，我们检测了紫外线照射小鼠和假照射小鼠肾上腺及血液中的多巴胺和去甲肾上腺素水平。我们发现，长期过度暴露于UVB会显著降低两种组织中的多巴胺水平，但不影响去甲肾上腺素水平。此外，UVB照射显著增加了相关酶酪氨酸羟化酶和多巴胺-β-羟化酶的水平。此外，我们还发现凋亡相关标志物增加，氧化防御蛋白减少，这可能导致了长期UVB照射小鼠肾上腺髓质明显的结构异常。这是长期暴露于UVB导致肾上腺损伤及随后儿茶酚胺代谢失调的首个证据。
