Chen Keda, Li Chaonan, Wang Ying, Shen Zhenwei, Guo Yikai, Li Xiaoping, Zhang Yanjun
Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China.
Department of Virus Inspection, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Vaccines (Basel). 2021 Jun 7;9(6):613. doi: 10.3390/vaccines9060613.
At present, there are no vaccines available for hand, foot, and mouth disease, which is caused by Coxsackie virus A16 (CVA16) infection. In the present study, we isolated epidemic strains of CVA16 and optimized the production of the virus in Vero cells. The system comprised growing the infected cells on polymer fiber paper carriers in a serum-free medium containing 0.5% (/) lactalbumin hydrolysate a mini bioreactor. Disposable Bioflo310 and AmProtein Current perfusion bioreactors were used to monitor virus infection and Vero cell culture. The total number of cells increased from 1.5 × 10 to 3.0 × 10. In our optimized culture process, the virus titer reached 7.8 × 10 TCID/mL at three days after infection. The inactivated CVA16 prepared from our optimized culture procedure elicited a slightly higher neutralizing antibody titer compared with that derived from routine culture procedures. These results will promote the large-scale production of inactivated CVA16 vaccines using nonwoven polymer fiber paper cell cultures.
目前，由柯萨奇病毒A16（CVA16）感染引起的手足口病尚无可用疫苗。在本研究中，我们分离出CVA16流行株并优化了其在Vero细胞中的生产。该系统包括在含有0.5%（/）水解乳白蛋白的无血清培养基中的聚合物纤维纸载体上培养感染细胞以及一个小型生物反应器。使用一次性Bioflo310和AmProtein连续灌注生物反应器监测病毒感染和Vero细胞培养。细胞总数从1.5×10增加到3.0×10。在我们优化的培养过程中，感染后三天病毒滴度达到7.8×10 TCID/mL。与常规培养程序制备的灭活CVA16相比，我们优化培养程序制备的灭活CVA16诱导产生的中和抗体滴度略高。这些结果将促进使用非织造聚合物纤维纸细胞培养大规模生产灭活CVA16疫苗。
