Mezzasalma Marcello, Odierna Gaetano, Petraccioli Agnese, Veith Michael, Guarino Fabio Maria
Department of Biology, University of Naples Federico II, I-80126 Naples, Italy.
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO, Universidade do Porto, Rua Padre Armando Quintas 7, 4485-661 Vairaõ, Portugal.
Animals (Basel). 2021 Jun 8;11(6):1709. doi: 10.3390/ani11061709.
We performed the first cytogenetic analysis on five out of the seven species of the genus , including seven subspecies, and representatives of its sister genus All the studied species have a similar karyotype of 2n = 24, mostly composed of biarmed elements. C-bands were observed on all chromosomes, at centromeric, telomeric and interstitial position. We found a peculiar taxon-specific NOR configuration, including either heteromorphic and homomorphic NORs on distinct regions of different chromosomes. and . showed two homomorphic NORs (pairs 8 and 2, respectively), while heteromorphic NORs were found in (pairs 6, 12), (pairs 2, 12), (pairs 2, 12), (pairs 6, 12), (pairs 2, 6) and (pairs 8, 10). Homomorphic NORs with an additional supernumerary site were shown by . . (pairs 2, 8) and . (pairs 2, 10). This unexpected highly variable NOR configuration is probably derived from multiple independent NOR translocations and paracentric inversions and correlated to lineage divergence in These results support the taxonomic validity of the studied taxa and are consistent with a hypothesized scenario of synchronous evolution in the genus.
我们对该属七个物种中的五个进行了首次细胞遗传学分析，包括七个亚种及其姐妹属的代表。所有研究物种的核型相似，均为2n = 24，主要由双臂染色体组成。在所有染色体的着丝粒、端粒和中间位置均观察到C带。我们发现了一种特殊的分类群特异性核仁组织区（NOR）构型，包括在不同染色体的不同区域上的异型和同型NOR。[具体物种1]和[具体物种2]分别在第8对和第2对染色体上显示出两个同型NOR，而异型NOR则在[具体物种3]（第6对、第12对）、[具体物种4]（第2对、第12对）、[具体物种5]（第2对、第12对）、[具体物种6]（第6对、第12对）、[具体物种7]（第2对、第6对）和[具体物种8]（第8对、第10对）中发现。[具体物种9]显示出具有额外超数位点的同型NOR。[具体物种10]（第2对、第8对）和[具体物种11]（第2对、第10对）也有此情况。这种意想不到的高度可变的NOR构型可能源自多个独立的NOR易位和臂内倒位，并与该属中的谱系分化相关。这些结果支持了所研究分类群的分类有效性，并与该属同步进化的假设情景一致。
