Department of Biomedical Engineering and Health Systems, Royal Technical Institute (KTH), Hälsovägen 11C, 141 27 Huddinge, Sweden.
Int J Mol Sci. 2021 Jun 8;22(12):6177. doi: 10.3390/ijms22126177.
Over the last few years, cryo electron microscopy has become the most important method in structural biology. While 80% of deposited maps are from single particle analysis, electron tomography has grown to become the second most important method. In particular sub-tomogram averaging has matured as a method, delivering structures between 2 and 5 Å from complexes in cells as well as in vitro complexes. While this resolution range is not standard, novel developments point toward a promising future. Here, we provide a guide for the workflow from sample to structure to gain insight into this emerging field.
在过去的几年中，冷冻电子显微镜已成为结构生物学中最重要的方法。虽然 80%的已储存图谱来自单颗粒分析，但电子断层扫描技术已发展成为第二重要的方法。特别是亚断层平均法已经成熟，可从细胞内以及体外复合物中获得 2 到 5Å 分辨率的结构。虽然这个分辨率范围不是标准的，但新的发展为这个新兴领域指明了充满希望的未来。在这里，我们提供了从样品到结构的工作流程指南，以深入了解这个领域。
