Redlarski Aleksander J, Klejdysz Tomasz, Kadej Marcin, Meyza Katarzyna, Vasilița Cristina, Oleksa Andrzej
Department of Genetics, Faculty of Biological Sciences, Kazimierz Wielki University, Powstańców Wielkopolskich 10, 85-090 Bydgoszcz, Poland.
Institute of Plant Protection-National Research Institute, Wegorka 20, 60-318 Poznan, Poland.
Insects. 2021 Jun 23;12(7):574. doi: 10.3390/insects12070574.
Obtaining biological material for DNA extraction is often challenging in organisms of conservation interest. Non-invasive sampling (i.e., sampling without injuring or disturbing an animal) is preferred as it carries no risk to the population's survival. Here, we tested the possibility of using the body remains left by bird predators for microsatellite genotyping in , a veteran oak specialist. We compared results obtained from such potentially degraded samples with samples of fresh beetle tarsi (i.e., invasive and destructive but non-lethal samples). Using 10 SSR loci, we genotyped 28 fresh, and 28 remains samples. The analysis indicated that PCR amplification efficiency was not influenced by sample type but allele length and individual heterozygosity. Allele frequencies were perfectly correlated for both sample types ( = 0.94). Additionally, null allele frequencies and genotyping failure rates were not significantly different from zero. Although the point estimates of individual inbreeding rates () were higher in remains than fresh samples (medians 0.08 vs. 0.02, respectively), both groups were not significantly different from each other and zero. Our study demonstrated that non-invasive remains samples could provide satisfactory data for population-genetic studies. However, we highlight the problem of biased inbreeding estimates, which may result from samples affected by allelic dropout.
对于具有保护价值的生物而言，获取用于DNA提取的生物材料往往具有挑战性。非侵入性采样（即不伤害或干扰动物的采样方式）是首选，因为它不会对种群的生存造成风险。在此，我们测试了利用鸟类捕食者留下的尸体残骸对资深橡树专家——进行微卫星基因分型的可能性。我们将从这些可能已降解的样本中获得的结果与新鲜甲虫跗节样本（即侵入性且具破坏性但非致命的样本）的结果进行了比较。利用10个简单序列重复（SSR）位点，我们对28个新鲜样本和28个残骸样本进行了基因分型。分析表明，聚合酶链反应（PCR）扩增效率不受样本类型影响，而是受等位基因长度和个体杂合性影响。两种样本类型的等位基因频率完全相关（ = 0.94）。此外，无效等位基因频率和基因分型失败率与零无显著差异。尽管残骸样本中个体近亲繁殖率（）的点估计值高于新鲜样本（中位数分别为0.08和0.02），但两组之间以及与零均无显著差异。我们的研究表明，非侵入性的残骸样本可为种群遗传学研究提供令人满意的数据。然而，我们强调了近亲繁殖估计存在偏差的问题，这可能是由受等位基因缺失影响的样本导致的。
