Tamoi Masahiro, Shigeoka Shigeru
Department of Advanced Bioscience, Faculty of Agriculture, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Agricultural Technology and Innovation Research Institute, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Plants (Basel). 2021 Jun 23;10(7):1275. doi: 10.3390/plants10071275.
We previously reported that CP12 formed a complex with GAPDH and PRK and regulated the activities of these enzymes and the Calvin-Benson cycle under dark conditions as the principal regulatory system in cyanobacteria. More interestingly, we found that the cyanobacterial CP12 gene-disrupted strain was more sensitive to photo-oxidative stresses such as under high light conditions and paraquat treatment. When a mutant strain that grew normally under low light was subjected to high light conditions, decreases in chlorophyll and photosynthetic activity were observed. Furthermore, a large amount of ROS was accumulated in the cells of the CP12 gene-disrupted strain. These data suggest that CP12 also functions under light conditions and may be involved in protection against oxidative stress by controlling the flow of electrons from Photosystem I to NADPH.
我们之前报道过，CP12在黑暗条件下作为蓝藻的主要调控系统，与甘油醛-3-磷酸脱氢酶（GAPDH）和磷酸核酮糖激酶（PRK）形成复合物，并调节这些酶的活性以及卡尔文-本森循环。更有趣的是，我们发现蓝藻CP12基因敲除菌株对高光条件和百草枯处理等光氧化胁迫更为敏感。当在低光条件下正常生长的突变菌株置于高光条件下时，观察到叶绿素含量和光合活性下降。此外，CP12基因敲除菌株的细胞中积累了大量活性氧（ROS）。这些数据表明，CP12在光照条件下也发挥作用，可能通过控制从光系统I到NADPH的电子流参与抗氧化胁迫。
