Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, Poland.
Proteomics and Mass Spectrometry Core Facility, Malopolska Centre of Biotechnology, Jagiellonian University, 31-007 Krakow, Poland.
Int J Mol Sci. 2021 Jun 23;22(13):6750. doi: 10.3390/ijms22136750.
High temperature stress leads to complex changes to plant functionality, which affects, i.a., the cell wall structure and the cell wall protein composition. In this study, the qualitative and quantitative changes in the cell wall proteome of leaves in response to high (40 °C) temperature stress were characterised. Using a proteomic analysis, 1533 non-redundant proteins were identified from which 338 cell wall proteins were distinguished. At a high temperature, we identified 46 differentially abundant proteins, and of these, 4 were over-accumulated and 42 were under-accumulated. The most significant changes were observed in the proteins acting on the cell wall polysaccharides, specifically, 2 over- and 12 under-accumulated proteins. Based on the qualitative analysis, one cell wall protein was identified that was uniquely present at 40 °C but was absent in the control and 24 proteins that were present in the control but were absent at 40 °C. Overall, the changes in the cell wall proteome at 40 °C suggest a lower protease activity, lignification and an expansion of the cell wall. These results offer a new insight into the changes in the cell wall proteome in response to high temperature.
高温胁迫会导致植物功能发生复杂变化，这会影响细胞壁结构和细胞壁蛋白组成。在这项研究中，我们对叶片在高温（40°C）胁迫下的细胞壁蛋白质组的定性和定量变化进行了研究。通过蛋白质组学分析，从 1533 个非冗余蛋白中鉴定出 338 种细胞壁蛋白。在高温下，我们鉴定出 46 种差异丰度蛋白，其中 4 种蛋白过度积累，42 种蛋白积累不足。观察到的最显著变化发生在作用于细胞壁多糖的蛋白质上，特别是 2 种过度积累的蛋白和 12 种积累不足的蛋白。基于定性分析，我们鉴定出一种在 40°C 下特异存在但在对照中不存在的细胞壁蛋白，以及在对照中存在但在 40°C 下不存在的 24 种细胞壁蛋白。总的来说，40°C 时细胞壁蛋白质组的变化表明蛋白酶活性降低、木质化和细胞壁扩张。这些结果为高温下细胞壁蛋白质组的变化提供了新的见解。
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