College of Life Science, Capital Normal University, 100048 Beijing, China.
Sci Rep. 2017 Apr 7;7:46183. doi: 10.1038/srep46183.
In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.
在这项研究中，我们使用拟南芥（Brachypodium distachyon L.）的根和叶，首次进行了对干旱胁迫响应和复水的综合生理和蛋白质组学分析。干旱胁迫导致叶片卷曲、根尖颜色变深，以及一些生理参数发生显著变化。二维差异凝胶电泳（2D-DIGE）鉴定了 78 个和 98 个差异积累蛋白（DAP）点，分别代表了根和叶中对干旱胁迫和/或复水响应的 68 个和 73 个独特蛋白。根和叶蛋白质组之间的差异最显著的是光合作用、能量代谢和蛋白质代谢。特别是，一些参与能量和蛋白质代谢的 DAP 在根和叶中的积累模式相反。根和叶的蛋白质-蛋白质相互作用（PPI）分析揭示了复杂的蛋白质相互作用网络，这些网络可以协同响应干旱胁迫和复水过程。使用实时定量聚合酶链反应（qRT-PCR）的转录分析验证了 PPI 网络中关键蛋白的差异表达。我们的综合生理和蛋白质组学分析为拟南芥根和叶中涉及干旱胁迫响应和复水过程的协同网络提供了证据。
