Walsh J P, Zhou F C, Hull C D, Fisher R S, Levine M S, Buchwald N A
Mental Retardation Research Center, University of California, Los Angeles 90024.
Synapse. 1988;2(1):37-44. doi: 10.1002/syn.890020107.
Physiological and morphological properties of transplanted striatal neurons (TSNs) were examined in an in vitro slice preparation. Fetal striatal tissue (E13-14) was implanted as a dissociated cellular suspension into the striatum of adult rats. Intracellular records were obtained from TSNs 2-6 weeks after transplantation. TSNs exhibited biophysical, morphological, and synaptic properties characteristic of normal striatal neurons, despite the disruption involved in processing of the fetal tissue. Differences were observed, however, between the TSNs and host striatal neurons. TSNs consistently had higher input resistance values than host striatal neurons as determined by neuronal responses to intracellular current injection. Stimulation of adjacent host striatum elicited both excitatory and inhibitory postsynaptic potentials in TSNs. By contrast, the same stimuli elicited only excitatory responses in host striatal neurons. Morphologically, TSNs resembled host medium-size spiny neurons as demonstrated by intracellular injection of lucifer yellow. However, the complexity of dendritic branching and the density of spines on the dendrites were less than that observed for host striatal neurons. It was concluded that during the posttransplantation period studied, TSNs possess neuronal properties expected of developmentally immature striatal neurons.
在体外脑片制备中检测了移植的纹状体神经元（TSNs）的生理和形态学特性。将胎龄13 - 14天的胎儿纹状体组织作为解离的细胞悬液植入成年大鼠的纹状体。在移植后2 - 6周从TSNs获得细胞内记录。尽管胎儿组织处理过程中存在干扰，但TSNs表现出正常纹状体神经元特有的生物物理、形态和突触特性。然而，在TSNs和宿主纹状体神经元之间观察到了差异。通过对细胞内电流注入的神经元反应确定，TSNs的输入电阻值始终高于宿主纹状体神经元。刺激相邻的宿主纹状体在TSNs中引发了兴奋性和抑制性突触后电位。相比之下，相同的刺激在宿主纹状体神经元中仅引发兴奋性反应。在形态上，通过荧光黄的细胞内注射证明，TSNs类似于宿主中等大小的棘状神经元。然而，树突分支的复杂性和树突上棘的密度低于宿主纹状体神经元。得出的结论是，在所研究的移植后时期，TSNs具有发育不成熟的纹状体神经元所预期的神经元特性。
