De Potter W P, Dillen L, Annaert W, Tombeur K, Berghmans R, Coen E P
Department of Medicine, University of Antwerp, Wilrijk, Belgium.
Synapse. 1988;2(2):157-62. doi: 10.1002/syn.890020207.
The subcellular localization and the secretion of neuropeptide Y were studied in sympathetic nerve endings of bovine vas deferens. Immunostaining revealed a parallel distribution for neuropeptide Y and dopamine-beta-hydroxylase immunoreactivity in the network of varicose nerve fibers in the smooth muscle layers of the vas deferens. Following differential centrifugation and sucrose density gradient centrifugation, neuropeptide Y was found to coincide with noradrenaline in the more dense region of the gradient, where the large dense cored vesicles are found. Superfusion experiments demonstrated the release of neuropeptide Y and noradrenaline upon electrical stimulation. Furthermore, the neuropeptide Y secretion was shown to be Ca2+-dependent. We conclude that in the bovine vas deferens neuropeptide Y is only present in large dense cored vesicles of adrenergic neurons and that the peptide and noradrenaline are co-released from these vesicles in a calcium dependent manner.
研究了牛输精管交感神经末梢中神经肽Y的亚细胞定位和分泌情况。免疫染色显示，在输精管平滑肌层曲张神经纤维网络中，神经肽Y和多巴胺-β-羟化酶免疫反应性呈平行分布。经过差速离心和蔗糖密度梯度离心后，发现神经肽Y与去甲肾上腺素在梯度的较致密区域重合，该区域存在大的致密核心囊泡。灌流实验表明，电刺激时神经肽Y和去甲肾上腺素会释放。此外，神经肽Y的分泌显示为Ca2+依赖性。我们得出结论，在牛输精管中，神经肽Y仅存在于肾上腺素能神经元的大的致密核心囊泡中，并且该肽和去甲肾上腺素以钙依赖的方式从这些囊泡中共同释放。
