Innes A, Power D A, Cunningham C, Dillon D, Catto G R
Department of Medicine & Therapeutics, University of Aberdeen, Foresterhill, Scotland.
Transplantation. 1988 Sep;46(3):409-13. doi: 10.1097/00007890-198809000-00016.
Maternal alloantibodies to paternal cells were monitored by cellular ELISA, the indirect hemagglutination and erythrocyte antibody rosette inhibition assays in sera and placental eluates from primigravid and multigravid inbred rats. In primigravid animals, antibodies in sera were routinely detected only by the indirect hemagglutination assay and were of low titer; weak antibody activity was detectable only by indirect hemagglutination in 1 of the 8 placental eluates assayed from these animals. Alloantibodies in high titer were present in sera and placental eluates from multigravid rats and were found to be directed predominantly to the RT1A (class I MHC) antigens of the paternal strain. These data provide no support for the hypothesis that the difficulty in detecting maternal antibodies during a 1st pregnancy is due to their preferential binding to antigenic determinants expressed on the placenta.
通过细胞酶联免疫吸附测定、间接血凝试验以及红细胞抗体玫瑰花结抑制试验，对初孕和经孕近交系大鼠血清及胎盘洗脱液中的母体抗父源细胞同种抗体进行了监测。在初孕动物中，血清中的抗体通常仅通过间接血凝试验检测到，且滴度较低；在这些动物检测的8份胎盘洗脱液中，只有1份通过间接血凝试验检测到微弱的抗体活性。经孕大鼠的血清和胎盘洗脱液中存在高滴度的同种抗体，且发现这些抗体主要针对父本品系的RT1A（I类主要组织相容性复合体）抗原。这些数据并不支持以下假说，即首次妊娠期间难以检测到母体抗体是由于它们优先结合胎盘上表达的抗原决定簇。
