从小鼠脾脏分离的细胞内细菌病原体的体内转录组分析。
Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Isolated from Mouse Spleen.
作者信息
Sun Na, Song Yanying, Liu Cong, Liu Mengda, Yu Lanping, Wang Fangkun
机构信息
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian 271018, China.
出版信息
Pathogens. 2021 Jun 30;10(7):823. doi: 10.3390/pathogens10070823.
() is an important intracellular pathogen that poses a health threat to humans. This study tries to clarify the mechanism of survival and reproduction in the host. In this study, high-throughput sequencing analysis was performed on RNA extracted from the strains isolated from infected mouse spleens and an reference strain (ATCC 14028) based on the BGISEQ-500 platform. A total of 1340 significant differentially expressed genes (DEGs) were screened. Functional annotation revealed DEGs associated with regulation, metabolism, transport and binding, pathogenesis, and motility. Through data mining and literature retrieval, 26 of the 58 upregulated DEGs (FPKM > 10) were not reported to be related to the adaptation to intracellular survival and were classified as candidate key genes (CKGs) for survival and proliferation in vivo. Our data contribute to our understanding of the mechanisms used by to regulate virulence gene expression whilst replicating inside mammalian cells.
（某病原体名称）是一种重要的细胞内病原体，对人类健康构成威胁。本研究试图阐明（该病原体名称）在宿主体内生存和繁殖的机制。在本研究中，基于BGISEQ - 500平台，对从感染小鼠脾脏分离的菌株以及一株参考菌株（ATCC 14028）提取的RNA进行了高通量测序分析。共筛选出1340个显著差异表达基因（DEGs）。功能注释显示DEGs与调控、代谢、转运和结合、致病机制及运动性相关。通过数据挖掘和文献检索，58个上调的DEGs（FPKM > 10）中有26个未被报道与细胞内存活适应相关，被归类为体内生存和增殖的候选关键基因（CKGs）。我们的数据有助于我们理解（该病原体名称）在哺乳动物细胞内复制时调节毒力基因表达所使用的机制。
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