Tan Zhigang, Chekabab Samuel M, Yu Hai, Yin Xianhua, Diarra Moussa S, Yang Chengbo, Gong Joshua
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
ACS Omega. 2019 Aug 7;4(8):13218-13230. doi: 10.1021/acsomega.9b01367. eCollection 2019 Aug 20.
The present study investigated the effects of iron, iron chelators, and mutations of or genes on the growth and virulence of Typhimurium Results indicated that organic iron (ferric citrate and ferrous-l-ascorbate) supported better growth of compared to inorganic iron. Among tested chelators, 2,2'-bipyridyl at 500 μM showed the highest inhibition of growth with 5 μM ferrous sulfate. Deletion of genes ( and ) in the iron uptake system attenuated invasion of Caco-2 cells and its ability to damage the epithelial monolayer. The expression of all tested host genes in Caco-2 was not affected under the iron-poor condition. However, claudin 3, tight junction protein 1, tumor necrosis factor α (TNF-α), and interleukin-8 (IL-8) were altered under the iron-rich condition depending on individual mutations. In , a significant down-regulation of ferritin 1 expression was observed when the nematode was infected by the wild-type (WT) strain.
本研究调查了铁、铁螯合剂以及 或 基因的突变对鼠伤寒沙门氏菌生长和毒力的影响。结果表明，与无机铁相比，有机铁（柠檬酸铁和亚铁 - l - 抗坏血酸盐）更有利于鼠伤寒沙门氏菌的生长。在测试的螯合剂中，500 μM 的 2,2'-联吡啶与 5 μM 硫酸亚铁一起时，对鼠伤寒沙门氏菌生长的抑制作用最强。铁摄取系统中基因（ 和 ）的缺失减弱了鼠伤寒沙门氏菌对 Caco - 2 细胞的侵袭及其破坏上皮单层的能力。在缺铁条件下，Caco - 2 中所有测试的宿主基因的表达均未受影响。然而，在富铁条件下，紧密连接蛋白 1、肿瘤坏死因子α（TNF -α）和白细胞介素 - 8（IL - 8）会因个体突变而发生改变。在 中，当线虫被野生型（WT）菌株感染时，观察到铁蛋白 1 表达显著下调。
