Sarasan Viswambharan, Pankhurst Tim, Yokoya Kazutomo, Sriskandarajah Sridevy, McDiarmid Faye
Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.
Plantlife, Brewery House, 36 Milford Street, Salisbury, Wiltshire SP1 2AP, UK.
Microorganisms. 2021 Jun 30;9(7):1421. doi: 10.3390/microorganisms9071421.
The yellow early marsh-orchid ( subsp. ) is critically endangered in the UK. Reintroduction of this threatened orchid to former haunts that have been restored is a long-term objective of this study. Identifying germination-specific mycorrhizal fungus lineages from closely related species is used as a method due to the extremely small number of plants left in the wild. A putative orchid mycorrhizal fungus of the family Tulasnellaceae, isolated from , supported in vitro seed germination to produce reintroduction-ready seedlings. Reintroduced symbiotic seedlings survived over the winter months in the flooded reintroduction site (RS). The comparative soil analysis for key nutrients before reintroduction showed that phosphorus content in the RS is very low compared to the soil collected from the wild site (WS) where the last viable population exists. On the other hand, C:N ratio in the soil at the WS and RS were not significantly different. To our knowledge, this is the first-ever report on the reintroduction of symbiotic seedlings of a threatened orchid back to the wild in the UK.
黄早生沼兰（亚种）在英国极度濒危。将这种受威胁的兰花重新引入已恢复的先前栖息地是本研究的长期目标。鉴于野外现存植株数量极少，从近缘物种中鉴定出特定于种子萌发的菌根真菌谱系作为一种方法。从[具体来源]分离出的一种推定的图拉斯内尔科兰花菌根真菌，支持种子在体外萌发以产生可用于重新引入的幼苗。重新引入的共生幼苗在被水淹没的重新引入地点（RS）度过了冬季。重新引入前对关键养分的土壤比较分析表明，与存在最后一个可存活种群的野生地点（WS）采集的土壤相比，RS中的磷含量非常低。另一方面，WS和RS土壤中的碳氮比没有显著差异。据我们所知，这是英国首次将受威胁兰花的共生幼苗重新引入野外的报告。
