Lin Yan, He Xiujuan, Xie Xinran, Liu Qingwu, Chen Jia, Li Ping
Beijing Institute of TCM, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, 23 Gallery Backstreet, Dongcheng District, Beijing 100010, China.
Beijing University of Chinese Medicine, No. 11, North Third Ring Road, Chaoyang District, Beijing 100029, China.
Evid Based Complement Alternat Med. 2021 Jun 9;2021:8881565. doi: 10.1155/2021/8881565. eCollection 2021.
Chronic nonhealing cutaneous wounds are a thorny problem in the field of surgery because of their prolonged and unhealed characteristics. Huiyang Shengji extract (HSE) is an extract of traditional Chinese medicine prescription for treating chronic wounds. This study aims to investigate the regulation of M1 macrophages on fibroblast proliferation and secretion and the intervention mechanism of Huiyang Shengji extract. We found that the effects of HSFs stimulated with paracrine factors from M1 macrophages were as follows: the proliferation of HSFs was reduced, the expression of MKI-67 was downregulated, and the content and gene expression of the inflammation factors and fibroblast MMPs were increased, while the content and gene expression of TIMP-1 are decreased, the content of human fibroblasts secreting type I collagen (COL1A1) and type III collagen (COL3A1) was decreased, and the TGF-1/Smad3 signaling pathway was inhibited. Interestingly, HSE inhibited these effects of M1 macrophages on human fibroblasts after the intervention, and the inhibitory effect was related to the concentration. In conclusion, M1 macrophages caused changes in HSFs and secretion, while HSE has a specific regulatory effect on the proliferation and secretion of fibroblasts caused by M1 macrophages.
慢性难愈性皮肤伤口因其病程长且不愈合的特点，成为外科领域的一个棘手问题。回阳生肌提取物（HSE）是一种用于治疗慢性伤口的中药方剂提取物。本研究旨在探讨M1巨噬细胞对成纤维细胞增殖和分泌的调控作用以及回阳生肌提取物的干预机制。我们发现，用M1巨噬细胞的旁分泌因子刺激人皮肤成纤维细胞（HSFs）后，其作用如下：HSFs的增殖减少，MKI-67的表达下调，炎症因子和成纤维细胞基质金属蛋白酶（MMPs）的含量及基因表达增加，而金属蛋白酶组织抑制因子-1（TIMP-1）的含量及基因表达降低，分泌I型胶原（COL1A1）和III型胶原（COL3A1）的人成纤维细胞含量减少，且转化生长因子-β1（TGF-β1）/Smad3信号通路受到抑制。有趣的是，干预后HSE抑制了M1巨噬细胞对人成纤维细胞的这些作用，且抑制作用与浓度有关。总之，M1巨噬细胞导致了HSFs及其分泌的变化，而HSE对M1巨噬细胞引起的成纤维细胞增殖和分泌具有特异性调节作用。
