London Centre for Nanotechnology, University College London, 17-19 Gordon Street, London WC1H 0AH, United Kingdom.
Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
J Synchrotron Radiat. 2021 Jul 1;28(Pt 4):1166-1173. doi: 10.1107/S1600577521004586. Epub 2021 Jun 23.
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
人类细胞核作为一个重要的细胞器，承载着生命的遗传蓝图。在这项工作中，应用了 X 射线叠层术，利用其独特的相移信息来评估人类细胞核的质量。测量是在英国钻石光源的 I13-1 光束线上进行的，该光束线具有非常大的横向相干特性。叠层衍射成像方法允许对大结构进行成像，从而对 2D 投影中的相移进行定量测量。本文提出了一种改进的叠层术算法，该算法可提高弱散射样品的重建质量。还展示了该方法在计算几个人类细胞核质量方面的应用。
