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[食品安全与质量控制成像技术的研究进展]

[Research advances in imaging technology for food safety and quality control].

作者信息

Deng Yamei, Wang Xiujuan, Yang Minli, He Muyi, Zhang Feng

机构信息

Chinese Academy of Inspection and Quarantine, Beijing 100176, China.

College of Pharmacy, China Medical University, Shenyang 110122, China.

出版信息

Se Pu. 2020 Jul 8;38(7):741-749. doi: 10.3724/SP.J.1123.2020.03015.

Abstract

Food quality and safety are issues of concern to the government, food industry, and consumers; hence, it is imperative to detect harmful substances in foodstuff. Traditional techniques for this purpose include biochemical methods and instrumental analysis methods such as chromatography and chromatography-mass spectrometry. These methods, however, are time-consuming and unable to obtain the spatial distribution of the analytes. Therefore, the development of rapid, non-destructive, real-time, and visual detection technologies has emerged as a hotspot in the field of food research. In recent years, hyperspectral imaging, which combines imaging and spectral technology, is rapidly gaining ground. This technique allows one to determine the geometrical characteristics and chemical composition of samples. Compared with traditional spectral technologies, hyperspectral imaging has the advantages of wide detection ranges, in addition to being real-time and non-destructive. At present, hyperspectral imaging is widely used in meat quality evaluation, detection of adulteration, and meat classification. In addition, Raman imaging is mainly used for the detection of illegal additives in food and for adulteration detection. This technology is fast, non-destructive, and low cost; furthermore, spectral and spatial information of the targets can be simultaneously obtained. Mass spectrometry imaging allows for the visualization and high-throughput analysis of sample tissues, without the need for complex sample preparation steps such as labeling and staining. Compared with other imaging technologies, mass spectrum information of substances can be obtained by mass spectrometry imaging. As a molecular visualized technology, it helps obtain the spatial distribution of nutrients and harmful substances in food. Mass spectrometry imaging has unique advantages in food research, e. g., it is used for molecular-level detection and accurate positioning of substances, and hence, it has excellent application prospects in this field. In this paper, recent literature data about imaging technologies in the field of food research, including 72 reports published in professional local and overseas magazines, are collated. The principles of hyperspectral imaging, Raman imaging, and mass spectrometry imaging are introduced, along with the detailed applications of these methods in the quality detection, source identification, and microbial pollution of food. In addition, it also includes food physical damage, food adulteration and food chemical residues. Besides, the advantages and disadvantages of these imaging technologies are discussed. Finally, prospects for the development of imaging technologies in food research are presented. Future work related to hyperspectral imaging should focus on the development of high-sensitivity cameras and high-resolution systems. Improving the data processing efficiency and adding prediction models are also key points for the future. Future studies on Raman imaging can focus on the application of different chemometrics algorithms that would improve the evaluation of food quality and safety parameters. Expanding the scope of application of these methods in food research will also be the focus of future research. Regarding mass spectrometry imaging, attempts should be made to improve the ionization methods, detection sensitivity, spatial resolution, and data processing effectiveness. Additionally, the combination of spectral imaging and mass spectrometry imaging gives full play to their advantages, so that spectral and mass spectrometry information of the targets can be obtained. In short, the application of imaging technologies in food research is expected to be more promising.

摘要

食品质量与安全是政府、食品行业和消费者关注的问题;因此,检测食品中的有害物质势在必行。为此目的的传统技术包括生化方法和仪器分析方法,如色谱法和色谱 - 质谱联用。然而,这些方法耗时且无法获得分析物的空间分布。因此,快速、无损、实时和可视化检测技术的发展已成为食品研究领域的热点。近年来,结合成像和光谱技术的高光谱成像迅速兴起。该技术能够确定样品的几何特征和化学成分。与传统光谱技术相比,高光谱成像除了具有实时性和无损性外,还具有检测范围广的优点。目前,高光谱成像广泛应用于肉质评价、掺假检测和肉类分类。此外,拉曼成像主要用于食品中非法添加剂的检测和掺假检测。该技术快速、无损且成本低;此外,还能同时获取目标的光谱和空间信息。质谱成像允许对样品组织进行可视化和高通量分析,无需诸如标记和染色等复杂的样品制备步骤。与其他成像技术相比,质谱成像能够获取物质的质谱信息。作为一种分子可视化技术,它有助于获得食品中营养成分和有害物质的空间分布。质谱成像在食品研究中具有独特优势,例如用于物质的分子水平检测和精确定位,因此在该领域具有良好的应用前景。本文整理了食品研究领域成像技术的近期文献数据,包括在国内外专业杂志上发表的72篇报告。介绍了高光谱成像、拉曼成像和质谱成像的原理,以及这些方法在食品质量检测、来源鉴定和微生物污染方面的详细应用。此外,还包括食品物理损伤、食品掺假和食品化学残留。此外,讨论了这些成像技术的优缺点。最后,展示了食品研究中成像技术的发展前景。与高光谱成像相关的未来工作应侧重于高灵敏度相机和高分辨率系统的开发。提高数据处理效率和添加预测模型也是未来的关键点。拉曼成像的未来研究可以侧重于不同化学计量学算法的应用,这将改善对食品质量和安全参数的评估。扩大这些方法在食品研究中的应用范围也将是未来研究的重点。关于质谱成像,应尝试改进电离方法、检测灵敏度、空间分辨率和数据处理效率。此外,光谱成像和质谱成像的结合充分发挥了它们的优势,从而可以获得目标的光谱和质谱信息。总之,成像技术在食品研究中的应用有望更具前景。

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