Animal & Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Grange, Co Meath, Ireland; Host-Pathogen Interactions Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Host-Pathogen Interactions Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.
Vet Immunol Immunopathol. 2021 Aug;238:110287. doi: 10.1016/j.vetimm.2021.110287. Epub 2021 Jun 17.
Interleukin-8 (IL-8) is an inflammatory chemokine released during the primary innate immune response to recruit neutrophils to the site of infection. Two distinct gene promoter haplotypes have been previously reported to segregate in the Holstein-Friesian breed (IL8-h1 and IL8-h2). Our earlier work showed how these divergent IL8 haplotypes influence IL-8 concentration and other immune response parameters at a systemic level. While a close relationship has been established between vitamin D and IL-8 in other species, the role of genetic haplotype on temporal variation in vitamin D concentrations and its impact on immunity remains unexplored in cattle. Therefore this study had two objectives - 1: to establish the temporal variation in IL-8 concentration profile in healthy calves of each IL-8 haplotype (n = 5/6 per group) and 2: to identify the relationship between systemic 25(OH)D concentration and IL8 haplotype in blood at 10 time points across their first year of life. Elevated IL-8 protein concentration profiles were apparent in IL8-h2 calves at multiple time points throughout the year (P < 0.05). In contrast, circulating concentrations of 25(OH) vitamin D were negatively correlated (0.38) with IL-8, with elevated concentrations in calves of the IL8-h1 haplotype. Increased numbers of innate immune cells - specifically monocytes and basophils, were also detected in blood from IL8-h2 calves (P < 0.05). Importantly, circulating concentrations of vitamin D were substantially below recommended concentrations of 30 ng/mL serum for optimal immunity in the first five months of life, indicating a window of potentially heightened disease susceptibility - particularly in calves of the IL8-h1 haplotype. In conclusion, this study has established that IL8 haplotype confers divergent chemokine concentrations and which contrasts with circulating concentrations of vitamin D. Accounting for both IL8 haplotype and vitamin D concentration may be critical to provide dairy calves with optimal immune protection in early life.
白细胞介素-8 (IL-8) 是一种炎症趋化因子，在原发性先天免疫反应中释放，以将中性粒细胞募集到感染部位。先前已经报道了两种不同的基因启动子单倍型在荷斯坦-弗里森牛品种中分离 (IL8-h1 和 IL8-h2)。我们之前的工作表明，这些不同的 IL8 单倍型如何在全身水平上影响 IL-8 浓度和其他免疫反应参数。虽然在其他物种中已经建立了维生素 D 和 IL-8 之间的密切关系，但遗传单倍型对维生素 D 浓度的时间变化及其对免疫的影响在牛中仍未得到探索。因此，本研究有两个目标-1：确定每个 IL-8 单倍型 (每组 5/6 头) 的健康小牛的 IL-8 浓度谱的时间变化，2：确定在它们生命的第一年的 10 个时间点，系统 25(OH)D 浓度与血液中 IL8 单倍型之间的关系。在整个一年中，IL8-h2 小牛的 IL-8 蛋白浓度升高（P <0.05）。相比之下，循环 25(OH) 维生素 D 浓度与 IL-8 呈负相关（0.38），IL8-h1 单倍型的小牛浓度升高。也在 IL8-h2 小牛的血液中检测到更多的先天免疫细胞-特别是单核细胞和嗜碱性粒细胞（P <0.05）。重要的是，在生命的前五个月，循环维生素 D 浓度远低于最佳免疫的推荐浓度 30ng/ml 血清，表明潜在的疾病易感性增加的窗口期-特别是在 IL8-h1 单倍型的小牛中。总之，本研究表明，IL8 单倍型赋予不同的趋化因子浓度，这与循环维生素 D 浓度形成对比。同时考虑 IL8 单倍型和维生素 D 浓度可能对为早期生命中的奶牛小牛提供最佳免疫保护至关重要。
