Research Centre for Animal Production and Aquaculture, Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Via Salaria, 31-Monterotondo, Rome, Italy.
Research Centre for Animal Production and Aquaculture, Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Via Salaria, 31-Monterotondo, Rome, Italy.
Vet Immunol Immunopathol. 2021 Aug;238:110288. doi: 10.1016/j.vetimm.2021.110288. Epub 2021 Jun 19.
Interleukin 8 (IL8) is a major mediator of the innate immune response. Polymorphisms in this gene are associated with susceptibility to inflammatory disease in humans. Two major promoter polymorphic haplotypes (IL8-h1 and IL8-h2) segregating in cattle populations have shown a significant effect on the immune response profile in calves but their implications for transition cow immunity have not been established. The aims of this study were to assess functional relevance of the IL8 haplotypes on the immunological traits of periparturient cows (n = 32) belonging to three genetic groups: Holstein (HO), Simmental (SI) and their crosses (CR) and to evaluate the frequency of IL8 haplotypes in the HO (dairy) and SI (dual purpose) pure breeds. IL8 haplotypes showed a significant effect on circulating number of both T helper lymphocytes (P = 0.0133) and T cytotoxic lymphocytes (P = 0.0024). Differences in percentage of CD14 monocytes and T lymphocyte subsets were found between haplotype groups at different time points. Plasma concentrations of Serum Amyloid A (SAA) and Haptoglobin (Hp) were enhanced at calving in IL8-h2 (P = 0.0019, P = 0.0029) and IL8-het (P = 0.050 and P = 0.052) respectively, compared with IL8-h1 cows. In contrast, significantly lower levels of reactive oxygen metabolites (d-ROMs) activation were identified in IL8-h2 and IL8-het cows after calving compared with IL8-h1 cows. Furthermore, genotyping results showed that SI cows have a high frequency of the homozygous IL8-h2 haplotype compared to the HO cows (87.5 % vs 40 %) which reflects the different selective pressure between the two pure breeds. In conclusion, our preliminary data suggests that IL8 promoter haplotype is associated with significant and dynamic changes in immunological traits during peripartum and early lactation period. Future work will focus on a more comprehensive assessment of immune changes in additional cows.
白细胞介素 8（IL8）是先天免疫反应的主要介质。该基因的多态性与人类炎症性疾病的易感性有关。在牛群中分离出的两个主要启动子多态单体型（IL8-h1 和 IL8-h2）对小牛的免疫反应谱有显著影响，但它们对过渡牛免疫力的影响尚未确定。本研究的目的是评估 IL8 单体型对属于三个遗传群体的围产期奶牛（n=32）的免疫特性的功能相关性：荷斯坦（HO）、西门塔尔（SI）及其杂交（CR），并评估 IL8 单体型在 HO（奶牛）和 SI（两用牛）纯系中的频率。IL8 单体型对循环 T 辅助淋巴细胞（P=0.0133）和 T 细胞毒性淋巴细胞（P=0.0024）的数量有显著影响。在不同时间点，不同单体型组之间发现 CD14 单核细胞和 T 淋巴细胞亚群的百分比存在差异。与 IL8-h1 奶牛相比，IL8-h2（P=0.0019，P=0.0029）和 IL8-het（P=0.050 和 P=0.052）在分娩时血清淀粉样蛋白 A（SAA）和触珠蛋白（Hp）的血浆浓度升高。相反，与 IL8-h1 奶牛相比，IL8-h2 和 IL8-het 奶牛在分娩后反应性氧代谢物（d-ROMs）的激活水平显著降低。此外，基因分型结果表明，与 HO 奶牛相比，SI 奶牛具有 IL8-h2 单体型的高同型合子频率（87.5%对 40%），这反映了两个纯系之间不同的选择压力。总之，我们的初步数据表明，IL8 启动子单体型与围产期和泌乳早期免疫特性的显著和动态变化有关。未来的工作将集中在对其他奶牛进行更全面的免疫变化评估。
