Gambarelli F, Dumon H
Laboratoire de Parasitologie, Université d'Aix-Marseille, Faculté de Médecine.
Ann Parasitol Hum Comp. 1988;63(2):160-2. doi: 10.1051/parasite/1988632160.
A rapid and simple technique for isolation of clones of Leishmania spp. was developed. This method consists to adapt promastigote forms of Leishmania infantum to an easily realized medium: the Panmede solid medium. We obtained cloned-population of this parasite for epidemiologic and toxonomic studies.
开发了一种快速简便的利什曼原虫属克隆分离技术。该方法包括将婴儿利什曼原虫的前鞭毛体形式适应于一种易于实现的培养基:泛培养基固体培养基。我们获得了这种寄生虫的克隆群体用于流行病学和分类学研究。