Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Israel; Department of Animal Science, The Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, Rehovot 76100, Israel.
Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Israel.
J Proteomics. 2021 Aug 30;246:104313. doi: 10.1016/j.jprot.2021.104313. Epub 2021 Jul 1.
We examined the effects of dietary n-3 fatty acids on the proteome of peripheral blood mononuclear cells (PBMC) in transition dairy cows. Forty-two dry cows were divided into three groups supplemented with: saturated fat (CTL); flaxseed oil (FLX); or fish oil (FO). PBMC were collected from five cows per group at week 1 postpartum for proteomic analysis. The n-3 fatty acid content in plasma and PBMC was higher in FLX and FO than in CTL cows. In PBMC, 3807 proteins were quantified and 44, 42 and 65 were differently abundant in FLX vs. CTL, FO vs. CTL and FLX vs. FO, respectively. In FLX vs. CTL, the abundance of the p65-subunit-of-transcription-factor NF-κB was higher, whereas albumin, C4b-binding protein and complement factor H levels were lower. In FLX vs. FO, complement factors B and H and hemopexin were higher. The top canonical pathway enriched in FLX compared to other groups was acute-phase-response signaling. The percentage of CD25+ blood cells was lower in FLX and FO at 1 week postpartum, and gene expression of NF-κB in white blood cells was lower in FLX than in CTL. Dietary sources of n-3 fatty acids differentially affected the proteome of PBMC, possibly altering the inflammatory status. SIGNIFICANCE: The transition dairy cow experiences a variable degree of systemic subacute inflammation, and proteomics of peripheral blood mononuclear cells (PBMC) may contribute to obtain insight into this process. Omega-3 fatty acids can moderate the immunological effect, and therefore we examined the effects of these fatty acids from flaxseed (FLX) or fish oils (FO) on the proteome of PBMC at week 1 postpartum. More than 3800 proteins were quantified, and in cows supplemented with FLX, enrichment of the acute-phase-signaling and complement systems were apparent in the PBMC compared to CTL and FO PBMC. This information may be useful to further explore the mechanism by which dietary omega-3 fatty acids affect the immune system in postpartum dairy cows.
我们研究了饮食中 n-3 脂肪酸对围产期奶牛外周血单核细胞（PBMC）蛋白质组的影响。将 42 头干奶牛分为三组，分别补充：饱和脂肪（CTL）；亚麻籽油（FLX）；或鱼油（FO）。产后第 1 周，每组从 5 头奶牛中采集 PBMC 进行蛋白质组分析。FLX 和 FO 组奶牛的血浆和 PBMC 中的 n-3 脂肪酸含量高于 CTL 组。在 PBMC 中，定量了 3807 种蛋白质，FLX 与 CTL 相比，44、42 和 65 种蛋白质丰度不同；FO 与 CTL 相比，FLX 与 FO 相比，44、42 和 65 种蛋白质丰度不同。FLX 与 CTL 相比，转录因子 NF-κB 的 p65 亚基丰度较高，而白蛋白、C4b 结合蛋白和补体因子 H 的水平较低。FLX 与 FO 相比，补体因子 B 和 H 以及血红素结合蛋白水平较高。与其他组相比，FLX 中富集的顶级经典途径是急性期反应信号。产后第 1 周，FLX 和 FO 组的 CD25+血细胞百分比较低，FLX 组白细胞中 NF-κB 的基因表达也低于 CTL 组。n-3 脂肪酸的饮食来源对 PBMC 的蛋白质组产生了不同的影响，可能改变了炎症状态。意义：围产期奶牛经历不同程度的全身亚急性炎症，外周血单核细胞（PBMC）的蛋白质组学可能有助于深入了解这一过程。ω-3 脂肪酸可以调节免疫作用，因此我们研究了亚麻籽油（FLX）或鱼油（FO）中的这些脂肪酸在产后第 1 周对 PBMC 蛋白质组的影响。定量了 3800 多种蛋白质，与 CTL 和 FO PBMC 相比，FLX 组奶牛的 PBMC 中急性期信号和补体系统的富集更为明显。这些信息可能有助于进一步探索膳食 ω-3 脂肪酸影响围产期奶牛免疫系统的机制。
