School of Pharmaceutical Science, University of South China, Hengyang, 421001, China; Guangzhou Institute of Respiratory Disease & China State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510182, China.
Guangzhou Institute of Respiratory Disease & China State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510182, China.
Biochem Biophys Res Commun. 2021 Sep 3;568:116-123. doi: 10.1016/j.bbrc.2021.06.075. Epub 2021 Jun 30.
Advanced CUBIC clearing method can clear diverse organs and even the entire body of a mouse to enable high-resolution 3D imaging. Advanced CUBIC reagent has often been used due to its low toxicity and easy preparation. However, Advanced CUBIC has a long experimental cycle, which reduces the efficiency of data acquisition. In this study, we first tracked the clarity changes of different organs cleared by Advanced CUBIC and identified the shortest time required for optimal transparency in individual organs. We then introduced ultrasound processing and developed a decolorization cocktail to optimize the clearing efficiency of the Advanced CUBIC method. With the optimized clearing CUBIC-Plus method, we achieved high resolution 3D imaging of mouse organs. Our CUBIC-Plus provides an efficient procedure to clear different organ for 3D imaging at high resolution.
高级 CUBIC 清除方法可以清除多种器官，甚至可以清除小鼠的整个身体，从而实现高分辨率 3D 成像。由于其低毒性和易于制备，高级 CUBIC 试剂经常被使用。然而，高级 CUBIC 具有较长的实验周期，这降低了数据采集的效率。在这项研究中，我们首先跟踪了不同器官经高级 CUBIC 清除后的清晰度变化，并确定了各个器官达到最佳透明度所需的最短时间。然后，我们引入了超声处理并开发了一种脱色鸡尾酒，以优化高级 CUBIC 方法的清除效率。通过优化的清除 CUBIC-Plus 方法，我们实现了对小鼠器官的高分辨率 3D 成像。我们的 CUBIC-Plus 为 3D 成像提供了一种高效的程序，可以清除不同的器官，以实现高分辨率。
