丙戊酸通过改变 MST1、Bcl-2 和 Nrf2 基因表达改善挫伤大鼠的运动功能。
Valproic Acid Ameliorates Locomotor Function in the Rat Model of Contusion via Alteration of Mst1, Bcl-2, and Nrf2 Gene Expression.
机构信息
Department of Molecular Medicine and Genetics, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran; 2Department of Anatomy, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran.
Department of Anatomy, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran.
出版信息
Iran Biomed J. 2021 Jul 1;25(4):303-7. doi: 10.52547/ibj.25.4.303.
BACKGROUND
In animal models of inflammatory diseases, Mammalian sterile 20-like kinase 1 (Mst1) facilitates the programmed cell death as a novel pro-apoptotic kinase. This research aimed to determine the expression level of Mst1 gene in a rat model of SCI treated with valproic acid (VPA).
METHODS
Severe rat model contusion was used for evaluation of the neuroprotective effect of valproic acid. The Basso-Beattie-Bresnahan test, was performed to determine locomotor functions. Hematoxylin/eosin staining and TUNEL assay were performed to detect cavity formation and apoptosis, respectively. The mRNA levels of the genes Mst1, nuclear factor (erythroid-derived 2)-like 2, and B-cell lymphoma 2 were evaluated, using quantitative real-time PCR acute spinal cord injury (RT-PCR).
RESULTS
The results revealed that Mst1 gene expression and TUNEL-positive cells in the VPA-treated group were significantly reduced as compared to the untreated group (p ≤ 0.05).
CONCLUSION
Our findings indicate that VPA has therapeutic potential and can be a candidate for the treatment of neurodegenerative disorders and traumatic injury as a promising drug.
背景
在炎症性疾病的动物模型中，哺乳动物 sterile 20-like kinase 1（Mst1）作为一种新型促凋亡激酶，促进程序性细胞死亡。本研究旨在确定用丙戊酸（VPA）治疗的 SCI 大鼠模型中 Mst1 基因的表达水平。
方法
采用严重大鼠挫伤模型评价丙戊酸的神经保护作用。进行 Basso-Beattie-Bresnahan 测试以确定运动功能。进行苏木精/伊红染色和 TUNEL 检测分别检测空洞形成和细胞凋亡。使用定量实时聚合酶链反应（RT-PCR）评估 Mst1、核因子（红细胞衍生 2）样 2 和 B 细胞淋巴瘤 2 基因的 mRNA 水平。
结果
结果表明，与未处理组相比，VPA 处理组的 Mst1 基因表达和 TUNEL 阳性细胞显著减少（p ≤ 0.05）。
结论
我们的研究结果表明，VPA 具有治疗潜力，可作为治疗神经退行性疾病和创伤性损伤的候选药物。
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