Yu B, Sawai T, Uehara M, Ishida T, Horiike K, Nozaki M
Department of Dermatology, Shiga University of Medical Science, Otsu, Japan.
Arch Dermatol. 1988 Oct;124(10):1530-3.
Using high-speed gel filtration chromatography, an aqueous extract of human dander was divided into four fractions (fractions I to IV). Scratch tests with the crude human dander extract and each fraction were performed in 147 patients with atopic dermatitis and 100 control subjects. The crude extract provoked positive reactions in 61.9% (91/147) of patients with atopic dermatitis and 6% (6/100) of control subjects. The most potent allergenic component was present in fraction II, which induced positive reactions in 61.2% (90/147) of patients with atopic dermatitis. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis demonstrated that fraction II contained two proteins with molecular weights of 10,000 and 13,000, respectively.
使用高速凝胶过滤色谱法，将人皮屑水提取物分为四个组分（组分I至IV）。用粗制人皮屑提取物和每个组分对147例特应性皮炎患者和100名对照受试者进行划痕试验。粗提取物在61.9%（91/147）的特应性皮炎患者和6%（6/100）的对照受试者中引发阳性反应。最具致敏性的成分存在于组分II中，其在61.2%（90/147）的特应性皮炎患者中诱导阳性反应。十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析表明，组分II包含两种分子量分别为10,000和13,000的蛋白质。
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