Assignment of the non-exchangeable proton resonances and solution conformation of the dodecanucleotide-d(CAATCCGGATTG).

The non-exchangeable base and sugar protons of the dodecanucleotide d(CAATCCGGATTG) have been assigned by the combined use of one and two-dimensional NMR spectroscopy. The sequential assignments of the base and sugar protons have been obtained using two dimensional nuclear Overhauser effect and homonuclear shift correlated spectra. By analysis of the nuclear Overhauser effect data it was determined that the dodecanucleotide assumes a right handed B-type helix in the aqueous medium used in this study.