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巴氏杀菌和高压处理对骆驼奶酪和牛乳奶酪品质的影响以及蛋白水解对骆驼奶酪柔软度的作用

Effects of Pasteurization and High-Pressure Processing of Camel and Bovine Cheese Quality, and Proteolysis Contribution to Camel Cheese Softness.

作者信息

Mbye Mustapha, Mohamed Huda, Ramachandran Tholkappiyan, Hamed Fathalla, AlHammadi Ahlam, Kamleh Rabih, Kamal-Eldin Afaf

机构信息

Department of Food Science, United Arab Emirates University, Al-Ain, United Arab Emirates.

Department of Physics, United Arab Emirates University, Al-Ain, United Arab Emirates.

出版信息

Front Nutr. 2021 Jun 17;8:642846. doi: 10.3389/fnut.2021.642846. eCollection 2021.

Abstract

The effects of high-pressure processing (HPP) compared to thermal treatments on the quality of camel vs. bovine cheeses were studied. The study showed that camel milk has a lower microbial load compared to bovine milk, which is maintained during 7 days' storage of the processed milk. The effect of three HPP treatments (350, 450, and 550 MPa for 5 min at 4°C) and two pasteurization treatments (65°C for 30 min and 75°C for 30 s) on the quality of soft unripened camel and bovine milk cheeses were accessed. The cheeses were evaluated for pH, yield, proximate composition, textural and rheological properties, microstructure, and protein profile by SDS-PAGE electrophoresis. The effects of the treatments on cheese's hardness were different between the camel and bovine cheeses; while heat treatment at 65°C for 30 min gave the hardest bovine milk cheese (1,253 ± 20), HPP treatment at 350 MPa for 5 min gave the highest value for camel milk cheese (519 ± 5) ( < 0.05). The hardness of the cheeses was associated with low yield and moisture content. SDS-PAGE electrophoresis revealed that extensive proteolysis might have contributed to the softness of camel cheeses compared to bovine and suggested the involvement of some residual enzyme activities.

摘要

研究了与热处理相比,高压处理(HPP)对骆驼奶酪和牛乳奶酪品质的影响。研究表明,与牛乳相比,骆驼乳的微生物负荷较低,且在加工后的牛奶储存7天期间保持不变。研究了三种高压处理(4℃下350、450和550MPa处理5分钟)和两种巴氏杀菌处理(65℃处理30分钟和75℃处理30秒)对未成熟软质骆驼乳和牛乳奶酪品质的影响。通过SDS-PAGE电泳对奶酪的pH值、产率、近似成分、质地和流变学特性、微观结构及蛋白质谱进行了评估。处理对骆驼奶酪和牛乳奶酪硬度的影响不同;虽然65℃热处理30分钟得到的牛乳奶酪硬度最高(1253±20),但350MPa高压处理5分钟得到的骆驼乳奶酪硬度值最高(519±5)(P<0.05)。奶酪的硬度与低产率和低水分含量有关。SDS-PAGE电泳显示,与牛乳奶酪相比,广泛的蛋白水解可能导致了骆驼奶酪的柔软度,并提示一些残留酶活性的参与。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba68/8248178/30270eac5a93/fnut-08-642846-g0001.jpg

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