Gibson Sophia B, Harper Clare S, Lackner Laura L, Andersen Erik C
Molecular Biosciences, Northwestern University, Evanston, IL, 60208, USA.
Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, 60208, USA.
MicroPubl Biol. 2021 Jun 30;2021. doi: 10.17912/micropub.biology.000411.
To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene.
为了更好地理解β-微管蛋白与苯并咪唑化合物之间假定相互作用所引起的抗性机制，我们试图在替换单个β-微管蛋白基因后通过异源表达来纯化线虫特异性β-微管蛋白。然而，我们发现含有线虫特异性β-微管蛋白基因的单倍体酵母细胞无法存活，这表明线虫β-微管蛋白不能替代酵母β-微管蛋白基因的缺失。
