Wang Y Y, Zhang Z R, Li J, Ren H L
Department of general surgery, Central Hospital of Zhumadian City, Zhu Madian 463000, China.
Department of Infectious Disease, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Gan Zang Bing Za Zhi. 2021 Jun 20;29(6):571-574. doi: 10.3760/cma.j.cn501113-20200620-00337.
To investigate the expression of miR-495 and its effect on MHCC-97H hepatocellular carcinoma cells. Fifty-six hepatocellular carcinoma tissue specimens (HCC group) and 40 normal liver tissue specimens (control group) preserved in our hospital from January 2017 to January 2018 were selected. Reverse transcription real-time fluorescent quantitative PCR (qRT-PCR) was used for miR-495 expression detection. MHCC-97H HCC cells were randomly selected and then divide into control group, blank plasmid group and transfection group. The blank plasmid group was transfected with blank plasmid, and the transfection group was transfected with miR-495 inhibitor. The expression of miR-495 in each group of cells were detected using qRT-PCR. CCK method was used to detect each group proliferation activity. Transwell cell migration assay was used to detect each group migration ability. Analysis of variance was used for comparison between multiple groups. Furthermore, LDS-t test was used for pairwise comparison, and t -test was used for comparison between the two groups. The relative expression levels of miR-495 in the HCC group was (2.043 ± 0.382), which was higher than the control group, and the difference between the two groups was statistically significant ( < 0.05). The relative expressions levels of miR-495 in patients with stage III to IV and lymph node metastasis were 2.265 ± 0.284 and 2.290 ± 0.355, which were significantly higher than those of stage I to II and no lymph node metastasis ( < 0.05). The relative expression levels of miR-495 in transfection group was 0.653 ± 0.102, which were significantly lower than control group and blank plasmid group ( < 0.05). The A values of MHCC-97H cells cultured for 24 h and 48 h in transfection group were 0.404 ± 0.106 and 0.604 ± 0.136, which were significantly lower than control group and blank plasmid group ( < 0.05). MHCC-97H cells migration number in the transfection group was (6.10 0 ± 20), which was significantly lower than that of control group and blank plasmid group ( < 0.05). miR-495 high expression has certain relationship with clinicopathological characteristics of HCC tissues. In addition, miR-495 has a certain effect on the proliferation and migration ability of MHCC-97H HCC cells.
探讨miR-495的表达及其对MHCC-97H肝癌细胞的影响。选取2017年1月至2018年1月我院保存的56例肝癌组织标本（肝癌组）和40例正常肝组织标本（对照组）。采用逆转录实时荧光定量PCR（qRT-PCR）检测miR-495表达。随机选取MHCC-97H肝癌细胞，分为对照组、空白质粒组和转染组。空白质粒组转染空白质粒，转染组转染miR-495抑制剂。采用qRT-PCR检测各组细胞中miR-495的表达。采用CCK法检测各组细胞增殖活性。采用Transwell细胞迁移实验检测各组细胞迁移能力。多组间比较采用方差分析。此外，两两比较采用LDS-t检验，两组间比较采用t检验。肝癌组miR-495相对表达水平为（2.043±0.382），高于对照组，两组间差异有统计学意义（<0.05）。ⅢⅣ期及有淋巴结转移患者miR-495相对表达水平分别为2.265±0.284和2.290±0.355，显著高于ⅠⅡ期及无淋巴结转移患者（<0.05）。转染组miR-495相对表达水平为0.653±0.102，显著低于对照组和空白质粒组（<0.05）。转染组培养24 h和48 h的MHCC-97H细胞A值分别为0.404±0.106和0.604±0.136，显著低于对照组和空白质粒组（<0.05）。转染组MHCC-97H细胞迁移数为（6.10±2.0），显著低于对照组和空白质粒组（<0.05）。miR-495高表达与肝癌组织的临床病理特征有一定关系。此外，miR-495对MHCC-97H肝癌细胞的增殖和迁移能力有一定影响。
