Ben Younes Ridha, Bouallegui Younes, Fezai Olfa, Mezni Amine, Touaylia Samir, Oueslati Ridha
Research Unit of Immuno-Microbiology, Environment and Carcinogenesis, Faculty of Sciences of Bizerte, University of Carthage, Tunisia.
LR01ES14 Laboratory of Environmental Biomonitoring, University of Carthage, Faculty of Sciences of Bizerte, Bizerte, Tunisia.
Drug Chem Toxicol. 2022 Sep;45(5):2371-2378. doi: 10.1080/01480545.2021.1945128. Epub 2021 Jul 6.
Silver nanoparticles (AgNPs) have been reported as stressors for the bivalves' immune system at different regulatory levels, impacting the detection step and receptors, and other mediators, as well as effector molecules. However, studies on how AgNPs impact the transmission of signals from receptors and whether they have an effect on mediators and transcription factors are still scarce. This study aims to investigate the effect of 12 hours of exposure to 100 µg/L of AgNPs on the gene expression of the cytosolic adaptor Myeloid, the differentiation protein 88 (MgMyD88-b), and the interferon regulatory factor (Me4-IRF) in the gills and digestive gland of before and after blocking two major uptake pathways of nanoparticles (clathrin- and caveolae-mediated endocytosis). The results illustrate a tissue-specific gene expression of the MgMyD88-b and the Me4-IRF in the gills and digestive gland of . In the gills, AgNPs did not significantly impact the expression of the two genes. However, blocking the caveolae-mediated endocytosis decreased the expression of Me4-IRF. However, inhibition of clathrin-mediated endocytosis in the digestive gland recorded a significant decrease in the expression of MgMyD88-b. Overall, the inhibition of the AgNPs' uptake routes have highlighted their potential interference with the immune response through the studied mediators' genes, which need to be studied further in future investigations.
据报道，银纳米颗粒（AgNPs）在不同调控水平上是双壳贝类免疫系统的应激源，会影响检测步骤、受体、其他介质以及效应分子。然而，关于AgNPs如何影响受体信号传递以及它们是否对介质和转录因子有影响的研究仍然很少。本研究旨在调查在阻断纳米颗粒的两种主要摄取途径（网格蛋白介导的内吞作用和小窝蛋白介导的内吞作用）前后，暴露于100μg/L AgNPs 12小时对鳃和消化腺中胞质衔接蛋白髓样分化蛋白88（MgMyD88-b）和干扰素调节因子（Me4-IRF）基因表达的影响。结果表明，MgMyD88-b和Me4-IRF在鳃和消化腺中存在组织特异性基因表达。在鳃中，AgNPs对这两个基因的表达没有显著影响。然而，阻断小窝蛋白介导的内吞作用会降低Me4-IRF的表达。然而，在消化腺中抑制网格蛋白介导的内吞作用记录到MgMyD88-b的表达显著降低。总体而言，抑制AgNPs的摄取途径突出了它们通过所研究的介质基因对免疫反应的潜在干扰，这需要在未来的研究中进一步探讨。
