Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Punjab, Pakistan.
Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan.
Curr Pharm Biotechnol. 2022;23(5):728-739. doi: 10.2174/1389201022666210702120956.
Raphanus sativus is traditionally used as an anti-inflammatory agent.
The current study was designed to explore the in vivo anti-inflammatory and antiangiogenic properties of Raphanus sativus seeds oil.
Cold press method was used for the extraction of oil (RsSO) and was characterised by using GC-MS techniques. Three in vitro antioxidant assays (DPPH, ABTS and FRAP) were performed to explore the antioxidant potential of RsSO. Disc diffusion methods were used to study in vitro antimicrobial properties. In vivo anti-inflammatory properties were studied in both acute and chronic inflammation models. In vivo chicken chorioallantoic membrane assay was performed to study antiangiogenic effects. Molecular mechanisms were identified using TNF-α ELISA kit and docking tools.
GC-MS analysis of RsSO revealed the presence of hexadecanoic and octadecanoic acid. Findings of DPPH, ABTS, and FRAP models indicated relatively moderate radical scavenging properties of RsSO. Oil showed antimicrobial activity against a variety of bacterial and fungal strains tested. Data of inflammation models showed significant (p < 0.05) anti-inflammatory effects of RsSO in both acute and chronic models. 500 mg/kg RsSO halted inflammation development significantly better (p < 0.05) as compared with lower doses. Histopathological evaluations of paws showed minimal infiltration of inflammatory cells in RsSO-treated animals. Findings of TNF-α ELSIA and docking studies showed that RsSO has the potential to down-regulate the expression of TNF-α, iNOS, ROS, and NF-κB respectively. Moreover, RsSO showed in vivo antiangiogenic effects.
Data of the current study highlight that Raphanus sativus seeds oil has anti-inflammatory, and antiangiogenic properties and can be used as an adjunct to standard NSAIDs therapy which may reduce the dose and related side effects.
萝卜历来被用作抗炎剂。
本研究旨在探讨萝卜籽油的体内抗炎和抗血管生成特性。
采用冷榨法提取油（RsSO），并采用 GC-MS 技术进行表征。采用 DPPH、ABTS 和 FRAP 三种体外抗氧化测定法（DPPH、ABTS 和 FRAP）来研究 RsSO 的抗氧化潜力。采用圆盘扩散法研究体外抗菌特性。在急性和慢性炎症模型中研究体内抗炎特性。体内鸡胚绒毛尿囊膜试验研究抗血管生成作用。采用 TNF-α ELISA 试剂盒和对接工具鉴定分子机制。
RsSO 的 GC-MS 分析表明存在十六烷酸和十八烷酸。DPPH、ABTS 和 FRAP 模型的结果表明 RsSO 具有相对中等的自由基清除能力。油对多种测试的细菌和真菌菌株表现出抗菌活性。炎症模型的数据表明，RsSO 在急性和慢性模型中均具有显著的抗炎作用（p<0.05）。与较低剂量相比，500mg/kg RsSO 能更显著地阻止炎症的发展（p<0.05）。对爪子的组织病理学评估显示，RsSO 处理的动物中炎性细胞浸润最小。TNF-α ELSIA 和对接研究的结果表明，RsSO 具有下调 TNF-α、iNOS、ROS 和 NF-κB 表达的潜力。此外，RsSO 显示出体内抗血管生成作用。
本研究的数据强调，萝卜籽油具有抗炎和抗血管生成特性，可以作为标准 NSAIDs 治疗的辅助手段，可能减少剂量和相关副作用。
