De Klerk G, Von dem Borne A E, Goudsmit R
Exp Hematol. 1978 Feb;6(2):193-200.
Using several immunological techniques, it was possible to demonstrate that toxicity of human serum in the foetal mouse liver cell bioassay for erythropoietin was due to complement-dependent IgM hetero antibodies to mouse foetal liver cells. The titre of the antibodies in 50 normal human sera ranged between 2 and 64 as measured in an agglutination test with mouse erythrocytes. Specificity of the antibodies for the ABO-or Ii-blood group system could not be established. Inactivation of complement by heating a serum for 30 minutes at 56 degrees C abolished toxicity.
