新生胎盘碱性磷酸酶的天冬氨酸-484与磷脂酰肌醇聚糖缩合，成为成熟酶的羧基末端。
Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.
作者信息
Micanovic R, Bailey C A, Brink L, Gerber L, Pan Y C, Hulmes J D, Udenfriend S
机构信息
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
出版信息
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1398-402. doi: 10.1073/pnas.85.5.1398.
Abstract
A carboxyl-terminal chymotryptic peptide from mature human placental alkaline phosphatase was purified by HPLC and monitored by a specific RIA. Sequencing and amino acid assay showed that the carboxyl terminus of the peptide was aspartic acid, representing residue 484 of the proenzyme as deduced from the corresponding cDNA. Further analysis of the peptide showed it to be a peptidoglycan containing one residue of ethanolamine, one residue of glucosamine, and two residues of neutral hexose. The inositol glycan is apparently linked to the alpha carboxyl group of the aspartic acid through the ethanolamine. Location of the inositol glycan on Asp-484 of the proenzyme indicates that a 29-residue peptide is cleaved from the nascent protein during the post-translational condensation with the phosphatidylinositol-glycan.
摘要
通过高效液相色谱法（HPLC）纯化了来自成熟人胎盘碱性磷酸酶的羧基末端胰凝乳蛋白酶肽段，并通过特异性放射免疫分析（RIA）进行监测。测序和氨基酸分析表明，该肽段的羧基末端为天冬氨酸，根据相应的cDNA推断，它代表酶原的第484位残基。对该肽段的进一步分析表明，它是一种肽聚糖，含有一个乙醇胺残基、一个氨基葡萄糖残基和两个中性己糖残基。肌醇聚糖显然通过乙醇胺与天冬氨酸的α羧基相连。肌醇聚糖在酶原的Asp-484上的定位表明，在与磷脂酰肌醇聚糖的翻译后缩合过程中，一个29个残基的肽段从新生蛋白质上被切割下来。
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Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.新生胎盘碱性磷酸酶的天冬氨酸-484与磷脂酰肌醇聚糖缩合，成为成熟酶的羧基末端。
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1398-402. doi: 10.1073/pnas.85.5.1398.
2
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本文引用的文献
1
Characterization of the Phospholipases of Bacillus cereus and Their Effects on Erythrocytes, Bone, and Kidney Cells.蜡样芽孢杆菌磷脂酶的特性及其对红细胞、骨骼和肾脏细胞的影响
J Bacteriol. 1965 Jul;90(1):69-81. doi: 10.1128/jb.90.1.69-81.1965.
2
Role of phosphatidylinositol in attachment of alkaline phosphatase to membranes.磷脂酰肌醇在碱性磷酸酶与膜结合中的作用。
Biochemistry. 1980 Aug 19;19(17):3913-8. doi: 10.1021/bi00558a004.
3
Rapid, automated analysis of monosaccharides by high-performance anion-exchange chromatography of borate complexes with fluorimetric detection using 2-cyanoacetamide.使用2-氰基乙酰胺通过硼酸盐络合物的高效阴离子交换色谱法结合荧光检测对单糖进行快速自动化分析。
Anal Biochem. 1981 May 1;113(1):130-8. doi: 10.1016/0003-2697(81)90055-5.
4
A gas-liquid solid phase peptide and protein sequenator.一种气-液-固相肽和蛋白质测序仪。
J Biol Chem. 1981 Aug 10;256(15):7990-7.
5
6
Microsequence analysis of peptides and proteins. II. Separation of amino acid phenylthiohydantoin derivatives by high-performance liquid chromatography on octadecylsilane supports.肽与蛋白质的微量序列分析。II. 用十八烷基硅烷载体上的高效液相色谱法分离氨基酸苯硫代乙内酰脲衍生物
Anal Biochem. 1982 Mar 1;120(2):302-11. doi: 10.1016/0003-2697(82)90351-7.
7
Purification and partial sequencing of human placental alkaline phosphatase.
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1076-83. doi: 10.1016/s0006-291x(83)80252-6.
8
Use of high-performance liquid chromatography for preparing samples for microsequencing.使用高效液相色谱法制备用于微量测序的样品。
J Chromatogr. 1984 Aug 3;297:13-9. doi: 10.1016/s0021-9673(01)89024-5.
9
High-performance liquid chromatography and picomole-level detection of peptides and proteins.肽和蛋白质的高效液相色谱及皮摩尔级检测
Methods Enzymol. 1981;79(Pt B):7-16. doi: 10.1016/s0076-6879(81)79006-2.
10
Amino acid analysis of proteins and peptides at the picomole level: the fluorescamine amino acid analyzer.皮摩尔水平蛋白质和肽的氨基酸分析：荧光胺氨基酸分析仪
Methods Enzymol. 1981;79(Pt B):20-5. doi: 10.1016/s0076-6879(81)79008-6.
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