Tianjin Key Laboratory of Molecular Optoelectronic, Department of Chemistry, Tianjin University, Tianjin 300072, People's Republic of China.
Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, People's Republic of China.
ACS Appl Mater Interfaces. 2021 Jul 21;13(28):32743-32752. doi: 10.1021/acsami.1c07101. Epub 2021 Jul 6.
In the past 10 years, many fluorescent probes have been developed to recognize G-quadruplexes (G4s) since G4s play an important role in biological systems. However, the selectivity and sensitivity of existing probes for G4s limit their further applications. Herein, we design and synthesize a new probe (TOVJ) by introducing 9-vinyljulolidine into TO. The new probe exhibits almost no fluorescence in an aqueous solution. Upon interacting with G4s, especially the antiparallel G4s, the fluorescence intensity was greatly enhanced (maximum 2742-fold) with a large Stokes shift of 198 nm and the maximum emission peak at 694 nm (near-infrared region). TOVJ showed high sensitivity and selectivity to G4s over other DNA topologies (ssDNA/dsDNA), especially to antiparallel G4s. For antiparallel human telomere G4 detection, the limits of detection of Hum24 and 22AG Na were as low as 164 and 231 pM, respectively. This indicates that TOVJ is a highly sensitive fluorescence sensor that can be effectively used for antiparallel human telomere G4 detection. The result of live-cell imaging showed that TOVJ could enter live cells and locate in the mitochondria.
在过去的 10 年中，已经开发出许多荧光探针来识别 G-四链体（G4s），因为 G4s 在生物系统中起着重要作用。然而，现有 G4s 探针的选择性和灵敏度限制了它们的进一步应用。在此，我们通过将 9-乙烯基乔利啶引入 TO 来设计和合成一种新的探针（TOVJ）。新探针在水溶液中几乎没有荧光。与 G4s 相互作用后，特别是与平行 G4s 相互作用后，荧光强度大大增强（最大增强 2742 倍），斯托克斯位移为 198nm，最大发射峰位于 694nm（近红外区）。TOVJ 对 G4s 具有高于其他 DNA 拓扑结构（ssDNA/dsDNA）的高灵敏度和选择性，特别是对平行 G4s。对于平行人类端粒 G4 的检测，Hum24 和 22AG Na 的检测限分别低至 164 和 231pM。这表明 TOVJ 是一种高灵敏度的荧光传感器，可有效用于平行人类端粒 G4 的检测。活细胞成像的结果表明，TOVJ 可以进入活细胞并定位在线粒体中。
